Sestero Christine M, Scalarone Gene M
Department of Biological Sciences, Idaho State University, Box 8007, Pocatello, ID 83209, USA.
Mycopathologia. 2006 Jul;162(1):33-7. doi: 10.1007/s11046-006-0028-7.
The objective of this study was to compare the efficacy of eight Blastomyces dermatitidis yeast phase lysate antigens (T-58: dog, Tennessee; T-27: polar bear, Tennessee; ERC-2: dog, Wisconsin; B5894: human, Minnesota; SOIL: soil, Canada; B5896: human, Minnesota; 48089: human, Zaire; 48938: bat, India) in the detection of the immunoglobulins IgG and IgM in serum specimens from canines with blastomycosis. An indirect enzyme-linked immunosorbent assay (ELISA, peroxidase system) was used to analyze sera collected during four different intervals post-infection. The yeast lysate antigen 48938 was a reactive antigen for the detection of both IgG (mean absorbance value range: 1.198-2.934) and IgM (mean absorbance value range: 0.505-0.845). For the same sera, antigen T-27 was also effective in the detection of IgG (mean absorbance value range: 0.904-3.356) and antigen 48089 was useful for the detection of IgM (mean absorbance value range: 0.377-0.554). The yeast lysate antigen B5894 proved to be a poor antigen for the detection of both IgG and IgM (mean absorbance value ranges: 0.310-0.744 for IgG, 0.025-0.069 for IgM). Inherent variations in yeast lysate antigens such as these may be utilized to develop improved immunoassay procedures for the specific detection of IgG or IgM in cases of blastomycosis.
本研究的目的是比较八种皮炎芽生菌酵母相裂解物抗原（T - 58：犬，田纳西州；T - 27：北极熊，田纳西州；ERC - 2：犬，威斯康星州；B5894：人，明尼苏达州；SOIL：土壤，加拿大；B5896：人，明尼苏达州；48089：人，扎伊尔；48938：蝙蝠，印度）在检测芽生菌病犬血清标本中免疫球蛋白IgG和IgM方面的效果。采用间接酶联免疫吸附测定法（ELISA，过氧化物酶系统）分析感染后四个不同时间段采集的血清。酵母裂解物抗原48938是检测IgG（平均吸光度值范围：1.198 - 2.934）和IgM（平均吸光度值范围：0.505 - 0.845）的反应性抗原。对于相同的血清，抗原T - 27在检测IgG（平均吸光度值范围：0.904 - 3.356）方面也有效，抗原48089在检测IgM（平均吸光度值范围：0.377 - 0.554）方面有用。酵母裂解物抗原B5894被证明是检测IgG和IgM的不良抗原（IgG的平均吸光度值范围：0.310 - 0.744，IgM的平均吸光度值范围：0.025 - 0.069）。诸如此类酵母裂解物抗原的内在差异可用于开发改进的免疫测定程序，以特异性检测芽生菌病病例中的IgG或IgM。
