Jarman S N, Deagle B E, Gales N J
Australian Antarctic Division, Channel Highway, Kingston, Tasmania 7050, Australia.
Mol Ecol. 2004 May;13(5):1313-22. doi: 10.1111/j.1365-294X.2004.02109.x.
Unique DNA sequences are present in all species and can be used as biomarkers for the detection of cells from that species. These DNA sequences can most easily be detected using the polymerase chain reaction (PCR), which allows very small quantities of target DNA sequence to be amplified even when the target is mixed with large amounts of nontarget DNA. PCR amplification of DNA markers that are present in a wide range of species has proven very useful for studies of species diversity in environmental samples. The taxonomic range of species to be identified from environmental samples may often need to be restricted to simplify downstream analyses and to ensure that less abundant sequences are amplified. Group-specific PCR primer sets are one means of specifying the range of taxa that produce an amplicon in a PCR. We have developed a range of group-specific PCR primers for studying the prey diversity found in predator stomach contents and scats. These primers, their design and their application to studying prey diversity and identity in predator diet are described.
所有物种都存在独特的DNA序列，这些序列可用作检测该物种细胞的生物标志物。使用聚合酶链反应（PCR）可以最容易地检测到这些DNA序列，即使靶标与大量非靶标DNA混合，PCR也能扩增极少量的靶标DNA序列。对广泛存在于多种物种中的DNA标记进行PCR扩增，已被证明对环境样品中的物种多样性研究非常有用。为简化下游分析并确保扩增较少丰度的序列，通常需要限制从环境样品中鉴定的物种分类范围。组特异性PCR引物组是一种指定在PCR中产生扩增子的分类单元范围的方法。我们开发了一系列组特异性PCR引物，用于研究捕食者胃内容物和粪便中的猎物多样性。本文描述了这些引物、它们的设计以及它们在研究捕食者饮食中猎物多样性和身份方面的应用。
