Chicote Eduardo, Moreno Diego A, Garcia Ana M, Sarro M Isabel, Lorenzo Petra I, Montero Felipe
Universidad Politécnica de Madrid, Departamento de Ingeniería y Ciencia de los Materiales, Escuela Técnica Superior de Ingenieros Industriales, José Gutiérrez Abascal 2, E-28006 Madrid, Spain.
Biofouling. 2004 Feb;20(1):35-42. doi: 10.1080/08927010410001662670.
Microbial activity in spent nuclear fuel pools which contain ultrapure and radioactive water has been previously observed. The aim of the present research was to isolate and identify the microorganisms attached to the nuclear pool wall of a Spanish nuclear power plant. Amplification of 16S rDNA fragments from the culturable microorganisms by PCR using universal primers for the domain 'Bacteria', followed by Denaturing Gradient Gel Electrophoresis analysis revealed the presence of six different bacteria. The complete gene for 16S rDNA of each one was sequenced and identified as belonging to three different phylogenetic groups, viz. beta-Proteobacteria, Actinomycetales and the Bacillus/Staphylococcus group. A fungus was also found and identified as Aspergillus fumigatus by sequencing the D2 region of the large subunit rDNA gene. The isolation of these microorganisms in oligotrophic and radioactive conditions is of great interest due to the possibility of their use in bioremediation processes of radionuclide-contaminated environments.
之前已观察到在含有超纯水和放射性水的乏核燃料池中存在微生物活动。本研究的目的是分离并鉴定附着在西班牙一座核电站核池壁上的微生物。使用针对“细菌”域的通用引物，通过聚合酶链反应（PCR）从可培养微生物中扩增16S rDNA片段，随后进行变性梯度凝胶电泳分析，结果显示存在六种不同的细菌。对每种细菌的16S rDNA完整基因进行测序，确定其属于三个不同的系统发育组，即β-变形菌纲、放线菌目和芽孢杆菌/葡萄球菌组。还发现了一种真菌，通过对大亚基rDNA基因的D2区域进行测序，鉴定为烟曲霉。在贫营养和放射性条件下分离出这些微生物，因其有可能用于放射性核素污染环境的生物修复过程而备受关注。
