Sarró M Isabel, García Ana M, Moreno Diego A
Department of Materials Science and Engineering, Industrial Engineers High Technical School, Technical University of Madrid, Spain.
Int Microbiol. 2005 Sep;8(3):223-30.
Microbiological studies of spent nuclear fuel pools at the Cofrentes Nuclear Power Plant (Valencia, Spain) were initiated to determine the microbial populations in the pools' water. Biofilm formation at the nuclear power plant facilities and the potential use of those microbial populations in the bioremediation of radioactive water were also studied. Biofilm formation was analyzed by immersing different austenitic stainless steel coupons (UNS S30400, UNS S30466, UNS S31600), as well as balls of stainless steel (UNS S44200) and titanium (99.9%) in a spent nuclear fuel pool (under static and dynamic conditions) for 34 months. Epifluorescence microscopy and scanning electron microscopy revealed that biofilm formed on the samples, in spite of the radioactive and oligotrophic conditions of the water. Based on standard culture methods and sequencing of 16S rDNA fragments, 57 bacteria belonging to alpha-, beta-, and gamma-Proteobacteria, Firmicutes and Actinobacteridae were identified in the biofilms. The radioactivity of the biofilm was measured using gamma-ray spectrometry, which revealed that biofilms were able to retain radionuclides, especially (60)Co. Using metallic materials to decontaminate radioactive water could become a new approach for bioremediation.
对西班牙巴伦西亚科夫雷核电站乏核燃料池开展了微生物学研究，以确定池水中的微生物种群。同时还研究了核电站设施中生物膜的形成情况以及这些微生物种群在放射性水生物修复中的潜在用途。通过将不同的奥氏体不锈钢试片（UNS S30400、UNS S30466、UNS S31600）以及不锈钢球（UNS S44200）和钛球（99.9%）在乏核燃料池中（在静态和动态条件下）浸泡34个月来分析生物膜的形成。落射荧光显微镜和扫描电子显微镜显示，尽管池水具有放射性且营养匮乏，但样品上仍形成了生物膜。基于标准培养方法和16S rDNA片段测序，在生物膜中鉴定出了57种属于α-、β-和γ-变形菌门、厚壁菌门和放线菌科的细菌。使用伽马射线光谱法测量了生物膜的放射性，结果表明生物膜能够保留放射性核素，尤其是钴-60。使用金属材料净化放射性水可能成为一种新的生物修复方法。
