Brien T P, Lanzafame R J, Naim J O, Rogers D W, Wang M J, Hinshaw J R
Laser Center, Rochester General Hospital, University of Rochester School of Medicine, New York 14621.
Lasers Surg Med. 1992;12(3):313-7. doi: 10.1002/lsm.1900120312.
This study examines whether primary laser excision results in augmentation of the systemic host anti-tumor response to tumor rechallenge. Single R3230AC mammary tumor implants, (0.5 x 0.5 x 1.0 mm), were grown in 112 female Fisher 344 rats. The animals were randomized. Group S tumors were excised by scalpel. Group E was excised with a Surgistat electrocautery (Valley Labs, Boulder, CO). Group CS was excised with a Sharplan 1100 CO2 laser (Sharplan, Allendale, NJ) at 25 watts (W) continuous wave (CW) (0.2 mm spot size) and the wound was "sterilized" with a 5-mm spot size by gently heating the tissue without blanching. Group K was excised with a KTP/532 laser (Laserscope, San Jose, CA) at 17 W CW using a 400 microns fiber. Group Y was excised with a Sharplan 2100 Nd:YAG laser set at 15W CW using a 0.2 mm clear sapphire tip. A second tumor implant, (0.5 x 0.5 x 1.0 mm), was placed at a remote site 14 days postoperatively. An unoperated control group was implanted. Secondary tumor volumes were measured for 36 days and the mean tumor volumes (MTV) were statistically compared. The MTV in groups CS, K, Y, and E was less than control (P less than 0.01). The MTV in groups CS, K, Y, and E was less than group S, although this was not statistically different. Lasers and cautery appear to increase the host response against subsequent tumor challenge. This study corroborates earlier studies of other modalities. Further studies to determine whether this host sensitization is an immune response and to elucidate the mechanisms of this effect are warranted.
本研究旨在探讨原发性激光切除是否会增强全身宿主对肿瘤再次攻击的抗肿瘤反应。将单个R3230AC乳腺肿瘤移植物（0.5×0.5×1.0毫米）植入112只雌性Fisher 344大鼠体内。动物被随机分组。S组肿瘤用手术刀切除。E组用Surgistat电灼器（Valley Labs，博尔德，科罗拉多州）切除。CS组用Sharplan 1100二氧化碳激光（Sharplan，阿伦代尔，新泽西州）以25瓦连续波（CW）（光斑尺寸0.2毫米）切除，通过在不使组织变白的情况下轻轻加热组织，用5毫米光斑尺寸对伤口进行“消毒”。K组用KTP/532激光（Laserscope，圣何塞，加利福尼亚州）以17瓦连续波使用400微米光纤切除。Y组用设置为15瓦连续波的Sharplan 2100钕:钇铝石榴石激光使用0.2毫米透明蓝宝石尖端切除。术后14天在远处部位植入第二个肿瘤移植物（0.5×0.5×1.0毫米）。植入一个未手术的对照组。测量继发性肿瘤体积36天，并对平均肿瘤体积（MTV）进行统计学比较。CS组、K组、Y组和E组的MTV小于对照组（P＜0.01）。CS组、K组、Y组和E组的MTV小于S组，尽管这在统计学上没有差异。激光和电灼似乎会增强宿主对后续肿瘤攻击的反应。本研究证实了早期对其他方式的研究。有必要进一步研究确定这种宿主致敏是否是一种免疫反应，并阐明这种效应的机制。
