Hyun Jinho, Kim Jeonghan, Craig Stephen L, Chilkoti Ashutosh
Department of Biomedical Engineering, Center for Biologically Inspired Materials and Material Systems, Duke University, Durham, North Carolina 27708-0281, USA.
J Am Chem Soc. 2004 Apr 21;126(15):4770-1. doi: 10.1021/ja049956q.
This paper describes a simple strategy to biochemically manipulate a surface at the nanoscale by enzyme dip-pen nanolithography using an endonuclease (DNase I) that is directly patterned on a self-assembled monolayer presenting a terminal oligonucleotide. Physisorbed nanopatterns of DNase I carried out nanoscale enzymology at the surface creating oligonucleotide patterns with the fidelity of the patterned enzyme because of the affinity of the enzyme for the immobilized, oligonucleotide substrate.
