利用核磁共振氢谱（¹H NMR）和多变量分析技术对野生型和转基因烟草植株进行代谢指纹图谱分析。

Metabolic fingerprinting of wild type and transgenic tobacco plants by 1H NMR and multivariate analysis technique.

作者信息

Choi Hyung-Kyoon, Choi Young Hae, Verberne Marianne, Lefeber Alfons W M, Erkelens Cornelis, Verpoorte Robert

机构信息

Department of Pharmacognosy, Section of Metabolomics, Institute of Biology, Leiden University, PO Box 9502, 2300 RA Leiden, The Netherlands.

出版信息

Phytochemistry. 2004 Apr;65(7):857-64. doi: 10.1016/j.phytochem.2004.01.019.

DOI:10.1016/j.phytochem.2004.01.019

PMID:15081285

Abstract

The metabolomic analysis of wild type and constitutive salicylic acid producing tobacco plants (CSA tobacco, Nicotiana tabacum 'Samsun' NN) plants overexpressing salicylate biosynthetic genes was carried out by 1H NMR spectrometry and multivariate analysis techniques. The principle component analysis (PCA) of the 1H NMR spectra showed a clear discrimination between those samples by PC1 and PC2. The discrimination of non-inoculated, TMV-virus inoculated, and systemic leaves or veins could also be obtained by PCA analysis. Major peaks in 1H NMR spectra contributing to the discrimination were assigned as those of chlorogenic acid, malic acid, and sugars. This method allows an efficient differentiation between wild type and transgenic plants without any pre-purification steps.

摘要

通过¹H NMR光谱法和多变量分析技术，对野生型烟草以及过表达水杨酸生物合成基因的组成型水杨酸产生烟草植株（CSA烟草，烟草品种‘Samsun’NN）进行了代谢组学分析。¹H NMR光谱的主成分分析（PCA）显示，PC1和PC2能清晰区分这些样品。通过PCA分析，也能区分未接种、接种烟草花叶病毒（TMV）的以及系统叶或叶脉。¹H NMR光谱中有助于区分的主要峰被确定为绿原酸、苹果酸和糖类的峰。该方法无需任何预纯化步骤就能有效区分野生型和转基因植株。