Zagorec M, Postma P W
E.C. Slater Institute for Biochemical Research, University of Amsterdam, The Netherlands.
Mol Gen Genet. 1992 Aug;234(2):325-8. doi: 10.1007/BF00283853.
The ptsG gene of Bacillus subtilis encodes Enzyme IIGlc of the phosphoenolpyruvate: glucose phosphotransferase system. The 3' end of the gene was previously cloned and the encoded polypeptide found to resemble the Enzymes IIIGlc of Escherichia coli and Salmonella typhimurium. We report here cloning of the complete ptsG gene of B. subtilis and determination of the nucleotide sequence of the 5' end. These results, combined with the sequence of the 3' end of the gene, revealed that ptsG encodes a protein consisting of 699 amino acids and which is similar to other Enzymes II. The N-terminal domain contains two small additional fragments, which share no similarities with the closely related Enzymes IIGlc and IINag of E. coli but which are present in the IIGlc-like protein encoded by the E. coli malX gene.
枯草芽孢杆菌的ptsG基因编码磷酸烯醇丙酮酸：葡萄糖磷酸转移酶系统的IIIGlc酶。该基因的3'端先前已被克隆，发现编码的多肽类似于大肠杆菌和鼠伤寒沙门氏菌的IIIGlc酶。我们在此报告枯草芽孢杆菌完整ptsG基因的克隆以及5'端核苷酸序列的测定。这些结果与该基因3'端的序列相结合，表明ptsG编码一种由699个氨基酸组成的蛋白质，并且与其他II类酶相似。N端结构域包含两个额外的小片段，它们与大肠杆菌密切相关的IIIGlc酶和IINag酶没有相似性，但存在于大肠杆菌malX基因编码的IIGlc样蛋白中。
