Gonzy-Tréboul G, Zagorec M, Rain-Guion M C, Steinmetz M
Institut Jacques Monod, Université Paris VII, France.
Mol Microbiol. 1989 Jan;3(1):103-12. doi: 10.1111/j.1365-2958.1989.tb00109.x.
The nucleotide sequence of a 1689bp fragment of the Bacillus subtilis locus containing ptsX (a crr-like gene), ptsH (coding for HPr), and the 5'-end of ptsI (coding for Enzyme I) was determined. The deduced amino acid sequences of ptsH and the N-terminal part of ptsI were compared to those of Streptococcus faecalis and Escherichia coli. Transcription fusion demonstrated that ptsHI constitutes an operon. An open reading frame overlapping the main part of ptsH in the opposite sense was shown to be expressed in vivo, using protein fusions with beta-galactosidase. The deduced amino acid sequence of ptsX showed significant homology with that of Salmonella typhimurium glucose-specific Enzyme III. ptsX was preceded by an open reading frame whose amino acid sequence showed strong homology with the C-terminal part of E. coli Enzyme IIGlc.
测定了枯草芽孢杆菌基因座一个1689bp片段的核苷酸序列，该片段包含ptsX（一个类crr基因）、ptsH（编码HPr）和ptsI（编码酶I）的5′端。将ptsH和ptsI N端部分推导的氨基酸序列与粪肠球菌和大肠杆菌的相应序列进行了比较。转录融合表明ptsHI构成一个操纵子。利用与β-半乳糖苷酶的蛋白融合，显示一个以相反方向与ptsH主要部分重叠的开放阅读框在体内表达。ptsX推导的氨基酸序列与鼠伤寒沙门氏菌葡萄糖特异性酶III的序列有显著同源性。ptsX之前有一个开放阅读框，其氨基酸序列与大肠杆菌酶IIGlc的C端部分有很强的同源性。
