Christiansen I, Hengstenberg W
Department of Microbiology, Ruhr-Universität Bochum, Germany.
Mol Gen Genet. 1996 Feb 25;250(3):375-9. doi: 10.1007/BF02174396.
Phosphoenolpyruvate (PEP)-dependent phosphorylation experiments have indicated that the gram-positive bacterium Staphylococcus carnosus possesses an EIICBA fusion protein specific for glucose. Here we report the cloning of a 7 kb genomic DNA fragment containing two genes, glcA and glcB, coding for the glucose-specific PTS transporters EII(Glc)1 and EII(Glc)2 which are 69% identical. The translation products derived from the nucleotide sequence consist of 675 and 692 amino acid residues and have calculated molecular weights of 73025 and 75256, respectively. Both genes can be stably maintained in Escherichia coli cells and restore the ability to ferment glucose to ptsG deletion mutants of E. coli. This demonstrates the ability of the PTS proteins HPr and/or EIIA(Glc) of a gram-negative organism (E. coli) to phosphorylate an EIICBA(Glc) from a gram-positive organism (S. carnosus).
磷酸烯醇丙酮酸（PEP）依赖性磷酸化实验表明，革兰氏阳性菌肉葡萄球菌拥有一种对葡萄糖具有特异性的EIICBA融合蛋白。在此，我们报告了一个7 kb基因组DNA片段的克隆，该片段包含两个基因，glcA和glcB，它们编码葡萄糖特异性磷酸转移酶系统转运蛋白EII(Glc)1和EII(Glc)2，二者的同一性为69%。从核苷酸序列推导的翻译产物分别由675和692个氨基酸残基组成，计算分子量分别为73025和75256。这两个基因均可在大肠杆菌细胞中稳定维持，并恢复大肠杆菌ptsG缺失突变体发酵葡萄糖的能力。这证明了革兰氏阴性菌（大肠杆菌）的磷酸转移酶系统蛋白HPr和/或EIIA(Glc)能够磷酸化革兰氏阳性菌（肉葡萄球菌）的EIICBA(Glc)。
