Cutler M L, Bassin R H, Zanoni L, Talbot N
Laboratory of Tumor Immunology and Biology, National Cancer Institute, Bethesda, Maryland 20892.
Mol Cell Biol. 1992 Sep;12(9):3750-6. doi: 10.1128/mcb.12.9.3750-3756.1992.
Using an expression cloning assay, we have isolated a novel cDNA, referred to as rsp-1, which suppresses the v-Ras-transformed phenotype. When introduced into NIH 3T3 fibroblasts under the control of a metallothionein promoter, rsp-1 confers resistance to v-Ras, but not to v-Mos or v-Src, and inhibits growth of the cells. The rsp-1 cDNA contains a 831-bp open reading frame encoding a 277-amino-acid leucine-rich protein. The rsp-1 cDNA exhibits no significant homology to sequences in the DNA data bases. However, searches of the protein data bases revealed that it contains a series of leucine-based repeats which are homologous to the leucine repeats found in the regulatory region of the yeast adenylyl cyclase. rsp-1 specific RNA is detectable in a wide variety of cell lines and tissues, and the gene is conserved among eukaryotic species. These data suggest that rsp-1 plays a role in Ras signal transduction.
利用表达克隆分析，我们分离出一种新型cDNA，称为rsp - 1，它能抑制v - Ras转化的表型。当在金属硫蛋白启动子的控制下导入NIH 3T3成纤维细胞时，rsp - 1赋予细胞对v - Ras的抗性，但对v - Mos或v - Src没有抗性，并抑制细胞生长。rsp - 1 cDNA包含一个831bp的开放阅读框，编码一个277个氨基酸的富含亮氨酸的蛋白质。rsp - 1 cDNA与DNA数据库中的序列没有明显同源性。然而，对蛋白质数据库的搜索显示，它包含一系列基于亮氨酸的重复序列，这些序列与酵母腺苷酸环化酶调节区域中发现的亮氨酸重复序列同源。在多种细胞系和组织中可检测到rsp - 1特异性RNA，并且该基因在真核生物物种中保守。这些数据表明rsp - 1在Ras信号转导中起作用。
