Koura Minako, Handa Hiroko, Noguchi Yoko, Takano Kaoru, Yamamoto Yoshie, Matsuda Junichiro, Suzuki Osamu
Department of Veterinary Science, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
Gen Comp Endocrinol. 2004 May 1;136(3):406-10. doi: 10.1016/j.ygcen.2004.01.012.
To examine the molecular basis for efficient superovulation in the Mongolian gerbil, the cDNA sequences of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) beta-subunits were determined and compared with those of other mammals. FSHbeta and LHbeta cDNAs were 1637 and 507bp long, respectively, from the 5'-end to putative polyA sites. The deduced sequences of the FSHbeta and LHbeta precursor proteins were 129 and 141 amino acids in length, respectively. The amino acid sequences of both Mongolian gerbil hormone subunits showed overall similarity to those of other rodents, confirming that the combination of equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) should be effective for induction of superovulation in Mongolian gerbils, as in mice and rats. However, the use of hCG might need to be re-evaluated owing to its low homology to rodent LH.
为了研究蒙古沙鼠高效超数排卵的分子基础，测定了促卵泡激素（FSH）和促黄体生成素（LH）β亚基的cDNA序列，并与其他哺乳动物的进行比较。从5′端到假定的聚腺苷酸位点，FSHβ和LHβ的cDNA分别长1637bp和507bp。推导的FSHβ和LHβ前体蛋白序列长度分别为129和141个氨基酸。蒙古沙鼠这两种激素亚基的氨基酸序列与其他啮齿动物的总体相似，证实马绒毛膜促性腺激素（eCG）和人绒毛膜促性腺激素（hCG）联合使用对诱导蒙古沙鼠超数排卵应该是有效的，就像在小鼠和大鼠中一样。然而，由于hCG与啮齿动物LH的同源性较低，其使用可能需要重新评估。
