Takano Kaoru, Koura Minako, Noguchi Yoko, Yamamoto Yoshie, Uchio-Yamada Kozue, Matsuda Junichiro, Suzuki Osamu
Department of Veterinary Science, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan.
Gen Comp Endocrinol. 2004 Sep 15;138(3):281-6. doi: 10.1016/j.ygcen.2004.06.009.
To examine the molecular basis of efficient superovulation in the Mastomys (Praomys coucha), the cDNA sequences of the follicle-stimulating hormone (FSH) and luteinizing hormone (LH) beta-subunits were determined and compared with those of other mammals. FSHbeta and LHbeta cDNAs were 1606 and 513 bp long, respectively, from the 5'-ends to the putative polyA sites. The deduced sequences of the FSHbeta and LHbeta precursor proteins were 130 and 141 amino acids in length, respectively. The amino acid sequences of both mastomys hormone subunits showed overall similarity to those of other rodents. In particular, the N-terminus of the FSHbeta precursor protein is of the MM-type, like those of mice and rats, which suggests that the MM-type is characteristic of the subfamily Murinae. As we reported earlier for the Mongolian gerbil, the use of hCG for superovulation of mastomys might need to be re-evaluated, due to the low homology between hCG and rodent LH sequences.
为研究非洲巨鼠（Coucha非洲巨鼠）高效超数排卵的分子基础，测定了促卵泡激素（FSH）和促黄体生成素（LH）β亚基的cDNA序列，并与其他哺乳动物的进行比较。从5′端到假定的多聚腺苷酸位点，FSHβ和LHβ的cDNA分别长1606和513bp。FSHβ和LHβ前体蛋白的推导序列长度分别为130和141个氨基酸。两种非洲巨鼠激素亚基的氨基酸序列与其他啮齿动物的总体相似。特别是，FSHβ前体蛋白的N端是MM型，与小鼠和大鼠的一样，这表明MM型是鼠科亚科的特征。正如我们之前对蒙古沙鼠的报道，由于hCG与啮齿动物LH序列之间的同源性较低，可能需要重新评估使用hCG对非洲巨鼠进行超数排卵的情况。
