Ryberg Henrik, Söderling Ann-Sofi, Davidsson Pia, Blennow Kaj, Caidahl Kenneth, Persson Lennart I
Institute of Clinical Neuroscience, Sahlgren University Hospital, University of Göteborg, 413 45 Göteborg, Sweden.
Neurochem Int. 2004 Jul;45(1):57-62. doi: 10.1016/j.neuint.2003.12.012.
The mechanisms behind the degeneration of neurons in diseases such as Alzheimer's disease (AD) and amyotrophic lateral sclerosis (ALS) are not fully understood. However, oxidation of certain amino acid residues in proteins may contribute to cell injury and some of these oxidized amino acids may also be suitable as biomarkers for oxidative injury. Therefore, it is suggested that the reaction between peroxynitrite (ONOO(-)) and tyrosine in vivo can be monitored by monitoring the formation of 3-nitrotyrosine (3-NT). In this work, a newly developed gas chromatographic-mass spectrometric method was applied to human cerebrospinal fluid (CSF). The free 3-NT levels were determined in the CSF from 19 controls, 17 patients with AD and 14 patients with ALS. The levels of free 3-NT in the CSF were considerably lower than those previously reported. The majority of the patients with AD or ALS had free 3-NT levels in the same range as seen in the control individuals and only a few patients showed increased levels of free 3-NT.
在阿尔茨海默病（AD）和肌萎缩侧索硬化症（ALS）等疾病中，神经元退化背后的机制尚未完全明确。然而，蛋白质中某些氨基酸残基的氧化可能导致细胞损伤，其中一些氧化氨基酸也可能适合作为氧化损伤的生物标志物。因此，有人提出，可通过监测3-硝基酪氨酸（3-NT）的形成来监测体内过氧亚硝酸根（ONOO(-)）与酪氨酸之间的反应。在这项研究中，一种新开发的气相色谱-质谱法被应用于人体脑脊液（CSF）。测定了19名对照者、17名AD患者和14名ALS患者脑脊液中的游离3-NT水平。脑脊液中游离3-NT的水平远低于先前报道的水平。大多数AD或ALS患者的游离3-NT水平与对照个体处于同一范围，只有少数患者的游离3-NT水平有所升高。
