Korolainen M A, Pirttilä T
Department of Neurology, Clinical Research Centre/Mediteknia, University of Kuopio and Kuopio University Hospital, Finland.
Acta Neurol Scand. 2009 Jan;119(1):32-8. doi: 10.1111/j.1600-0404.2008.01057.x. Epub 2008 Jun 10.
Many studies have shown differences in carbonylation and nitration of individual proteins in brain and body fluids of Alzheimer's disease (AD) patients. Therefore, we wanted to examine whether total levels of these oxidative stress markers of proteins were altered in AD.
Total levels of carbonyls and nitrotyrosine in cerebrospinal fluid, serum and plasma were measured in 22 AD patients and 18 age-matched controls using commercially available enzyme immunoassay kits.
Protein carbonylation in cerebrospinal fluid did not differ between AD patients and controls but was decreased in APOE epsilon4 carriers as compared with non-carriers. Serum but not plasma levels of carbonyls tended to be decreased in AD patients as compared with aged controls. Nitrotyrosine concentrations did not differ between the groups. Surrogate cerebrospinal fluid markers for AD, beta-amyloid (1-42) and tau, correlated with blood carbonyl and nitrotyrosine levels.
According to these preliminary data, changes in oxidative metabolism related to the pathogenesis of AD cannot be detected as increased cerebrospinal fluid, serum or plasma protein carbonylation or nitration.
许多研究表明，阿尔茨海默病（AD）患者脑和体液中个别蛋白质的羰基化和硝化存在差异。因此，我们想研究这些蛋白质氧化应激标志物的总水平在AD中是否发生改变。
使用市售酶免疫分析试剂盒，对22例AD患者和18例年龄匹配的对照者的脑脊液、血清和血浆中的羰基和硝基酪氨酸总水平进行了测量。
AD患者和对照者脑脊液中的蛋白质羰基化无差异，但与非携带者相比，APOE ε4携带者中的蛋白质羰基化降低。与老年对照者相比，AD患者血清而非血浆中的羰基水平有降低趋势。各组间硝基酪氨酸浓度无差异。AD的替代脑脊液标志物β-淀粉样蛋白（1-42）和tau与血液羰基和硝基酪氨酸水平相关。
根据这些初步数据，无法检测到与AD发病机制相关的氧化代谢变化表现为脑脊液、血清或血浆中蛋白质羰基化或硝化增加。
