Mancini M, Ricci A, Amenta F
Dipartimento di Sanità Pubblica e Biologia Cellulare, Università Tor Vergata, Roma, Italy.
Neurobiol Aging. 1992 Jul-Aug;13(4):501-4. doi: 10.1016/0197-4580(92)90078-c.
The density and distribution of sulfide-silver staining in the neostriatum of 3-, 12-, and 24-month-old male Sprague-Dawley rats were analyzed using the neo-Timm sulfide-silver histochemical technique associated with microdensitometry. This technique stains zinc-containing terminals in the striatum and the density of neo-Timm staining is considered to be parallel to the density of synaptic boutons containing zinc. In the neostriatum sulfide-silver, staining was intense in the matrix, although the striosomes did not show appreciable reactivity. The density of sulfide-silver staining was significantly reduced (p less than 0.001) in the matrix of 12-month-old in comparison to 3-month-old rats. No further changes were noticeable between 24- and 12-month-old rats. In contrast, the area and the perimeter of neostriatum that were assessed by quantitative image analysis did not show age-related changes. The present results indicated that similar to the observations for a variety of neurochemical parameters of rat neostriatum such as local cerebral glucose utilization, cholinergic muscarinic receptors, and dopamine D-1 receptors, zinc-containing striatal terminal were primarily decreased between young and adult subjects but not between adult and aged animals.
采用与显微密度测定法相关的新Timm硫化银组织化学技术，分析了3个月、12个月和24个月大的雄性Sprague-Dawley大鼠新纹状体中硫化银染色的密度和分布。该技术可对纹状体中含锌终末进行染色，新Timm染色的密度被认为与含锌突触小体的密度平行。在新纹状体中，硫化银染色在基质中强烈，尽管纹状体小体未显示出明显的反应性。与3个月大的大鼠相比，12个月大的大鼠基质中硫化银染色的密度显著降低（p<0.001）。在24个月和12个月大的大鼠之间未观察到进一步变化。相比之下，通过定量图像分析评估的新纹状体面积和周长未显示出与年龄相关的变化。目前的结果表明，与大鼠新纹状体的各种神经化学参数（如局部脑葡萄糖利用、胆碱能毒蕈碱受体和多巴胺D-1受体）的观察结果相似，含锌纹状体终末在年轻和成年个体之间主要减少，但在成年和老年动物之间没有减少。
