Esmekaya Meric Arda, Kayhan Handan, Coskun Alaaddin, Canseven Ayse G
Department of Biophysics, Faculty of Medicine, Gazi University, 06510, Beşevler, Ankara, Turkey.
Department of Internal Medicine, Division of Hematology, Faculty of Medicine, Gazi University, Ankara, Turkey.
J Membr Biol. 2016 Oct;249(5):601-610. doi: 10.1007/s00232-016-9891-4. Epub 2016 Mar 28.
Electrochemotherapy is the usage of electroporation to introduce chemotherapeutic drugs through membrane pores into target cells for cancer treatment. The effectiveness of chemotherapeutic drugs would be increased dramatically when they are used in electrochemotherapy than standard chemotherapy. In the present study, we investigated the effects of cisplatin treatment with electroporation on human SH-SY5Y neuroblastoma cells. SH-SY5Y cells were treated with different concentrations (0.15-24 µg/mL) of cisplatin and then exposed to 1500 volts per centimeter (V/cm), 100 microseconds (µs) pulse duration, and 1 Hertz (Hz) electric pulses. Cisplatin alone showed a dose-dependent effect on cell viability. On the other hand, cisplatin + electroporation treatment was more effective than cisplatin treatment alone. Lower doses of cisplatin treatment with electroporation was as effective as higher doses of cisplatin treatment without electroporation. These results indicated that cisplatin cytotoxicity was potentiated after exposure of cells to high intensity electric pulses and low doses of cisplatin can be used with electroporation in the treatment of neuroblastoma.
电化学疗法是利用电穿孔技术将化疗药物通过细胞膜孔导入靶细胞以治疗癌症。与标准化疗相比，化疗药物在电化学疗法中的疗效会显著提高。在本研究中，我们研究了顺铂电穿孔处理对人SH-SY5Y神经母细胞瘤细胞的影响。用不同浓度（0.15 - 24μg/mL）的顺铂处理SH-SY5Y细胞，然后施加1500伏/厘米（V/cm）、100微秒（µs）脉冲持续时间和1赫兹（Hz）的电脉冲。单独使用顺铂对细胞活力呈剂量依赖性影响。另一方面，顺铂 + 电穿孔处理比单独使用顺铂治疗更有效。低剂量顺铂电穿孔处理与高剂量顺铂非电穿孔处理效果相当。这些结果表明，细胞暴露于高强度电脉冲后顺铂细胞毒性增强，低剂量顺铂可与电穿孔联合用于神经母细胞瘤的治疗。
