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同源重组是如何起始的：来自V(D)J位点特异性重组中单链切口的意外证据。

How homologous recombination is initiated: unexpected evidence for single-strand nicks from v(d)j site-specific recombination.

作者信息

Smith Gerald R

机构信息

Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA.

出版信息

Cell. 2004 Apr 16;117(2):146-8. doi: 10.1016/s0092-8674(04)00338-1.

DOI:10.1016/s0092-8674(04)00338-1
PMID:15084252
Abstract

Views of how homologous recombination is initiated have changed over the past several decades: in the 1960s and 1970s, single-strand DNA lesions (nicks) were the leading contenders, but in the last decade, double-strand DNA breaks (DSBs) have reigned. In this issue of Cell, evidence is presented that nicks can stimulate homologous recombination and, in uncontrolled situations, may lead to translocations and other potentially dangerous genome rearrangements.

摘要

在过去几十年里，关于同源重组如何起始的观点发生了变化：在20世纪60年代和70年代，单链DNA损伤（切口）是主要的候选因素，但在过去十年中，双链DNA断裂（DSB）占据了主导地位。在本期《细胞》杂志中，有证据表明切口可以刺激同源重组，并且在不受控制的情况下，可能导致易位和其他潜在危险的基因组重排。

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How homologous recombination is initiated: unexpected evidence for single-strand nicks from v(d)j site-specific recombination.同源重组是如何起始的：来自V(D)J位点特异性重组中单链切口的意外证据。
Cell. 2004 Apr 16;117(2):146-8. doi: 10.1016/s0092-8674(04)00338-1.
2
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Double-strand signal sequence breaks in V(D)J recombination are blunt, 5'-phosphorylated, RAG-dependent, and cell cycle regulated.V(D)J重组中的双链信号序列断裂是平端的、5'-磷酸化的、依赖RAG的且受细胞周期调控。
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The DDE motif in RAG-1 is contributed in trans to a single active site that catalyzes the nicking and transesterification steps of V(D)J recombination.RAG-1中的DDE基序以反式作用于一个单一的活性位点，该位点催化V(D)J重组的切口和转酯步骤。
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Both V(D)J recombination and radioresistance require DNA-PK kinase activity, though minimal levels suffice for V(D)J recombination.V(D)J重组和辐射抗性都需要DNA-PK激酶活性，不过V(D)J重组所需的活性水平很低。
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