Gellert M
Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.
Semin Immunol. 1994 Jun;6(3):125-30. doi: 10.1006/smim.1994.1018.
Several lines of evidence have suggested the existence of broken DNA molecules as intermediates in V(D)J recombination. These include sequence alterations at the recombined junctions and the presence of aberrant recombinants. More recently, broken DNA molecules have been directly detected in recombinationally active cells. The signal sequence ends have normal blunt-ended DNA breaks, but the coding ends have a hairpin (self-joined) structure that provides an explanation for the self-complementary P nucleotide insertions often found after V(D)J joining in the antigen receptor genes.
多条证据表明，断裂的DNA分子作为中间体存在于V(D)J重组过程中。这些证据包括重组连接点处的序列改变以及异常重组体的存在。最近，在具有重组活性的细胞中已直接检测到断裂的DNA分子。信号序列末端具有正常的平端DNA断裂，但编码末端具有发夹（自身连接）结构，这为抗原受体基因V(D)J连接后经常发现的自身互补P核苷酸插入现象提供了解释。
