高静水压对选定微小核糖核酸病毒的灭活作用
Inactivation of selected picornaviruses by high hydrostatic pressure.
作者信息
Kingsley David H, Chen Haiqiang, Hoover Dallas G
机构信息
Microbial Food Safety Research Unit, U.S. Department of Agriculture, Agricultural Research Service, James W. W. Baker Center, Delaware State University, Dover, DE 19901, USA.
出版信息
Virus Res. 2004 Jun 15;102(2):221-4. doi: 10.1016/j.virusres.2004.01.030.
The potential of high hydrostatic pressure processing (HPP) to inactivate Aichi virus (AiV), human parechovirus-1 (HPeV-1), and the coxsackievirus strains A9 and B5 was investigated. For coxsackievirus A9 (CAV9), a 5-min HPP treatment in minimum essential growth medium (MEM) supplemented with 10% fetal bovine sera (FBS) resulted in 3.4-, 6.5-, and 7.6-log(10) tissue culture infectious dose (50%) (TCID(50)) reductions at 400, 500, and 600megaPascals (MPa), respectively. For HPeV-1, a 5-min treatment in MEM with 10% FBS resulted in reductions of 1.3-, 4.3-, and 4.6-log(10) TCID(50) at 400, 500, and 600MPa, respectively; however, AiV and coxsackievirus B5 (CBV5) in MEM supplemented with 2 and 10% FBS, respectively, remained fully infectious after a 5-min treatment at 600MPa. These data establish that different picornaviruses have widely variable pressure inactivation thresholds in response to HPP.
研究了高静水压处理（HPP）对爱知病毒（AiV）、人细小病毒1型（HPeV-1）以及柯萨奇病毒A9和B5株的灭活潜力。对于柯萨奇病毒A9（CAV9），在补充有10%胎牛血清（FBS）的最低必需生长培养基（MEM）中进行5分钟的HPP处理，在400、500和600兆帕斯卡（MPa）时分别导致3.4、6.5和7.6对数（10）组织培养感染剂量（50%）（TCID（50））降低。对于HPeV-1，在含有10%FBS的MEM中进行5分钟处理，在400、500和600MPa时分别导致1.3、4.3和4.6对数（10）TCID（50）降低；然而，分别在补充有2%和10%FBS的MEM中的AiV和柯萨奇病毒B5（CBV5）在600MPa下处理5分钟后仍具有完全传染性。这些数据表明，不同的微小核糖核酸病毒对HPP的压力灭活阈值差异很大。
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