Kingsley David H, Chen Haiqiang, Hoover Dallas G
Microbial Food Safety Research Unit, U.S. Department of Agriculture, Agricultural Research Service, James W. W. Baker Center, Delaware State University, Dover, DE 19901, USA.
Virus Res. 2004 Jun 15;102(2):221-4. doi: 10.1016/j.virusres.2004.01.030.
The potential of high hydrostatic pressure processing (HPP) to inactivate Aichi virus (AiV), human parechovirus-1 (HPeV-1), and the coxsackievirus strains A9 and B5 was investigated. For coxsackievirus A9 (CAV9), a 5-min HPP treatment in minimum essential growth medium (MEM) supplemented with 10% fetal bovine sera (FBS) resulted in 3.4-, 6.5-, and 7.6-log(10) tissue culture infectious dose (50%) (TCID(50)) reductions at 400, 500, and 600megaPascals (MPa), respectively. For HPeV-1, a 5-min treatment in MEM with 10% FBS resulted in reductions of 1.3-, 4.3-, and 4.6-log(10) TCID(50) at 400, 500, and 600MPa, respectively; however, AiV and coxsackievirus B5 (CBV5) in MEM supplemented with 2 and 10% FBS, respectively, remained fully infectious after a 5-min treatment at 600MPa. These data establish that different picornaviruses have widely variable pressure inactivation thresholds in response to HPP.
研究了高静水压处理（HPP）对爱知病毒（AiV）、人细小病毒1型（HPeV-1）以及柯萨奇病毒A9和B5株的灭活潜力。对于柯萨奇病毒A9（CAV9），在补充有10%胎牛血清（FBS）的最低必需生长培养基（MEM）中进行5分钟的HPP处理，在400、500和600兆帕斯卡（MPa）时分别导致3.4、6.5和7.6对数（10）组织培养感染剂量（50%）（TCID（50））降低。对于HPeV-1，在含有10%FBS的MEM中进行5分钟处理，在400、500和600MPa时分别导致1.3、4.3和4.6对数（10）TCID（50）降低；然而，分别在补充有2%和10%FBS的MEM中的AiV和柯萨奇病毒B5（CBV5）在600MPa下处理5分钟后仍具有完全传染性。这些数据表明，不同的微小核糖核酸病毒对HPP的压力灭活阈值差异很大。
