一种使用纤维蛋白固定的蓝色葡聚糖测定人血浆中纤溶酶和纤溶酶抑制剂活性的方法。
A method using fibrin-fixed Blue Dextran for determining the plasmin and plasmin inhibitor activities in human plasma.
作者信息
Tamura Y, Otsuka A, Sone K, Sakurai E, Mori H, Sumi H, Kosugi T, Fujii S
出版信息
Biochim Biophys Acta. 1978 Jul 7;525(1):194-9. doi: 10.1016/0005-2744(78)90214-0.
The fibrinolytic activity of plasmin was determined by incubating with fibrin-fixed Blue Dextran as a substrate, the Blue Dextran released being proportional to the plasmin activity. The applicability of this method for rapid and accurate evaluation of fibrinolytic activity was demonstrated by dose-response curves with purified plasmin, plasmin generated by urokinase in human plasma and euglobulin. The method can also be used to determined plasmin inhibitors in plasma.
通过与固定在纤维蛋白上的蓝色葡聚糖作为底物孵育来测定纤溶酶的纤溶活性，释放出的蓝色葡聚糖与纤溶酶活性成正比。用纯化的纤溶酶、尿激酶在人血浆中产生的纤溶酶以及优球蛋白的剂量反应曲线证明了该方法在快速准确评估纤溶活性方面的适用性。该方法还可用于测定血浆中的纤溶酶抑制剂。
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