Nahamura Hideji, Yoshida Kenya, Kishima Yoshihiko, Enomoto Hirayuki, Uyama Hirokazu, Kuroda Toshifumi, Okuda Yorihide, Hirotani Tomonori, Ito Hiroaki, Kawase Ichiro
Department of Molecular Medicine, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.
Hepatogastroenterology. 2004 Mar-Apr;51(56):470-5.
BACKGROUND/AIMS: Various circulating auto-antibodies have been reported in patients with ulcerative colitis. Hepatoma-derived growth factor (HDGF) is a mitogen, localized dominantly in the nucleus of proliferating cells. In this study, we demonstrated the circulating anti-HDGF auto-antibody and investigated its clinical roles in patients with ulcerative colitis.
Anti-HDGF IgG antibodies were measured by the enzyme-linked immunosorbent assay with recombinant HDGF in 20 healthy volunteers and 40 patients with ulcerative colitis.
Circulating anti-HDGF antibody was detected in the serum of a patient with total colitis by Western blotting. Anti-HDGF auto-antibodies were detected at 65.6% in the serum of patients with total/left-sided colitis, compared with healthy subjects at 10%. During active stage, the circulating anti-HDGF auto-antibodies were detected at a higher frequency of 78.3% than those in remission stage at 37.5%. Furthermore, the titers during active colitis were higher than those during the remission stage. Anti-HDGF auto-antibodies were not detected in any patients with proctitis.
These findings suggest that anti-HDGF auto-antibodies in the serum of patients with ulcerative colitis would help to classify the total/left-sided colitis from proctitis, and the serial measurement of the titer would also be a good marker for the active colitis.
背景/目的：溃疡性结肠炎患者中已报道存在多种循环自身抗体。肝癌衍生生长因子（HDGF）是一种促分裂原，主要定位于增殖细胞核内。在本研究中，我们证实了循环抗HDGF自身抗体，并研究了其在溃疡性结肠炎患者中的临床作用。
采用酶联免疫吸附测定法，用重组HDGF检测20名健康志愿者和40例溃疡性结肠炎患者血清中的抗HDGF IgG抗体。
通过蛋白质印迹法在全结肠炎患者血清中检测到循环抗HDGF抗体。全结肠炎/左侧结肠炎患者血清中抗HDGF自身抗体的检出率为65.6%，而健康受试者为10%。在活动期，循环抗HDGF自身抗体的检出频率为78.3%，高于缓解期的37.5%。此外，活动期结肠炎时的抗体滴度高于缓解期。在任何直肠炎患者中均未检测到抗HDGF自身抗体。
这些发现表明，溃疡性结肠炎患者血清中的抗HDGF自身抗体有助于将全结肠炎/左侧结肠炎与直肠炎区分开来，抗体滴度的系列检测也将是活动期结肠炎的良好标志物。
