Craig Stephen, Laming Peter
The Queen's University Belfast, School of Biology and Biochemistry, 97 Lisburn Road, Belfast BT9 7BL, Northern Ireland, UK.
Water Res. 2004 Apr;38(8):2144-54. doi: 10.1016/j.watres.2004.01.021.
The validity of using an automated online biomonitoring system, the multispecies freshwater biomonitor (MFB), in recording the physiological and behavioural activities of the three-spined stickleback Gasterosteous aculeatus, is assessed. The direct impact of the alternating electrical current generated by the impedance converter on fish activity was measured using a repeated measures design, machine on/machine off. Twelve subjects were used in each of three experimental groups: (1). standard hard water environment (control), (2). 0.1mM NH(4)Cl and (3). 10mM NH(4)Cl. Impedance generated waves were compared to corresponding manually recorded behavioural observations in an attempt to calibrate the MFB, assigning particular behaviours to a particular frequency and amplitude range. Data analysis suggests that the MFB current did not affect fish behaviour at any of the three contamination levels studied. Furthermore, analysis of the frequency with which behavioural activity changed (activity phases classified as inactive or active) showed that MFB behavioural data was not significantly different from that recorded manually. This suggested that the MFB recorded the behaviours that were concurrently observed. The feasibility of using the MFB as an effective behavioural early warning system in an aquacultural environment and as a scientific tool for toxicity testing is discussed.
评估了使用自动化在线生物监测系统——多物种淡水生物监测仪（MFB）记录三刺鱼（Gasterosteous aculeatus）生理和行为活动的有效性。使用重复测量设计（机器开启/机器关闭）测量了阻抗转换器产生的交流电对鱼类活动的直接影响。三个实验组每组使用12只受试鱼：（1）标准硬水环境（对照组），（2）0.1mM氯化铵，（3）10mM氯化铵。将阻抗产生的波形与相应的手动记录行为观察结果进行比较，试图校准MFB，将特定行为与特定频率和振幅范围相关联。数据分析表明，在所研究的三个污染水平中的任何一个水平下，MFB电流均未影响鱼类行为。此外，对行为活动变化频率（活动阶段分为不活跃或活跃）的分析表明，MFB行为数据与手动记录的数据无显著差异。这表明MFB记录了同时观察到的行为。讨论了将MFB用作水产养殖环境中有效的行为预警系统以及用作毒性测试科学工具的可行性。
