急性高同型半胱氨酸血症和轻度高甘油三酯血症期间健康成年人的内皮细胞粘附分子

Endothelial cell adhesion molecules in healthy adults during acute hyperhomocysteinemia and mild hypertriglyceridemia.

作者信息

Mansoor M A, Seljeflot I, Arnesen H, Knudsen A, Bates C J, Mishra G, Larsen T W

机构信息

Division of Medical Biochemistry, Rogaland Central Hospital, 4068 Stavanger, Norway.

出版信息

Clin Biochem. 2004 May;37(5):408-14. doi: 10.1016/j.clinbiochem.2004.01.003.

DOI:10.1016/j.clinbiochem.2004.01.003

PMID:15087258

Abstract

OBJECTIVE

Hyperhomocysteinemia and hypertriglyceridemia are independently associated with atherosclerotic disease. The process of atherogenesis involves inflammation and endothelial dysfunction. We tested whether concurrent acute hyperhomocysteinemia and mild hypertriglyceridemia increase the concentrations of circulatory cellular adhesion molecules in healthy subjects.

STUDY DESIGN AND METHODS

Twelve healthy volunteers aged 37.5 years (range, 25-51) participated in the present study. The concentrations of plasma total homocysteine (p-tHcy), serum triglycerides, circulatory cellular adhesion molecules (CAMs), and concentrations of nitrate were measured at 0 (fasting), 2, 4, and 6 h after loading with (1) methionine, (2) fat, (3) methionine + fat, and (4) water (control). Wash out period between each loading was >or=1 week.

RESULTS

Percent relative changes from baseline in the concentrations of p-tHcy, 2, 4, and 6 h after methionine and methionine + fat were significantly different from after water and fat loading. Changes in the concentrations of serum triglycerides 2 h after fat loading were significantly different from water loading, whereas methionine + fat loading caused a significant difference after 2, 4, and 6 h. We detected a synergistic increase in the triglyceride area response to methionine + fat loading. We detected also a significant difference in percent relative changes in the concentrations of P-selectin (PSEL) (P = 0.02), E-selectin (ESEL) (P = 0.003), and vascular cell adhesion molecule-1 (VCAM-1) (P = 0.005) 6 h after methionine + fat loading as compared to water loading. There was an additive increase in the PSEL area response to methionine + fat loading. Furthermore, area response to VCAM was greater to methionine loading than water loading (P = 0.01). A decrease in the concentration of NO(3) was more pronounced after methionine + fat loading and a significantly decreased area response of nitrate to methionine + fat loading was detected than to area response to water loading (P = 0.002).

CONCLUSION

Inflammatory activation of the endothelium takes place during concurrent transient hyperhomocysteinemia and mild hypertriglyceridemia.

摘要

目的

高同型半胱氨酸血症和高甘油三酯血症均与动脉粥样硬化性疾病独立相关。动脉粥样硬化的形成过程涉及炎症和内皮功能障碍。我们检测了在健康受试者中，同时存在的急性高同型半胱氨酸血症和轻度高甘油三酯血症是否会增加循环中细胞黏附分子的浓度。

研究设计与方法

12名年龄37.5岁（范围25 - 51岁）的健康志愿者参与了本研究。在空腹时（0小时）以及在给予（1）蛋氨酸、（2）脂肪、（3）蛋氨酸 + 脂肪和（4）水（对照）后2、4和6小时，测量血浆总同型半胱氨酸（p - tHcy）、血清甘油三酯、循环细胞黏附分子（CAMs）以及硝酸盐的浓度。每次给予之间的洗脱期≥1周。

结果

蛋氨酸和蛋氨酸 + 脂肪负荷后2、4和6小时，p - tHcy浓度相对于基线的百分比变化与给予水和脂肪负荷后有显著差异。脂肪负荷后2小时血清甘油三酯浓度的变化与给予水后有显著差异，而蛋氨酸 + 脂肪负荷在2、4和6小时后导致显著差异。我们检测到对蛋氨酸 + 脂肪负荷的甘油三酯面积反应有协同增加。我们还检测到，与给予水相比，蛋氨酸 + 脂肪负荷后6小时，P选择素（PSEL）（P = 0.02）、E选择素（ESEL）（P = 0.003）和血管细胞黏附分子 - 1（VCAM - 1）（P = 0.005）浓度的相对变化百分比有显著差异。对蛋氨酸 + 脂肪负荷的PSEL面积反应有相加增加。此外，对VCAM的面积反应，蛋氨酸负荷比水负荷更大（P = 0.01）。蛋氨酸 + 脂肪负荷后硝酸盐浓度的降低更明显，并且检测到硝酸盐对蛋氨酸 + 脂肪负荷的面积反应比水负荷的面积反应显著降低（P = 0.002）。