使用核酸外切酶III对PCR产物进行定向克隆。 - Suppr

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超能文献

Directional cloning of PCR products using exonuclease III.

作者信息

Kaluz S, Kölble K, Reid K B

机构信息

Department of Biochemistry, University of Oxford, UK.

出版信息

Nucleic Acids Res. 1992 Aug 25;20(16):4369-70. doi: 10.1093/nar/20.16.4369.

DOI:10.1093/nar/20.16.4369

PMID:1508730

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC334153/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c0a4/334153/ba918d5e726c/nar00227-0245-a.jpg

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本文引用的文献

Unidirectional digestion with exonuclease III in DNA sequence analysis.DNA序列分析中用核酸外切酶III进行单向消化。

Methods Enzymol. 1987;155:156-65. doi: 10.1016/0076-6879(87)55014-5.

Cloning of PCR products after defined cohesive termini are created with T4 DNA polymerase.用T4 DNA聚合酶产生特定粘性末端后对PCR产物进行克隆。

Nucleic Acids Res. 1990 Jul 25;18(14):4290. doi: 10.1093/nar/18.14.4290.

Efficient cloning of PCR generated DNA containing terminal restriction endonuclease recognition sites.高效克隆含末端限制性内切酶识别位点的PCR扩增DNA。

Nucleic Acids Res. 1990 Oct 25;18(20):6156. doi: 10.1093/nar/18.20.6156.

Functional cloning vectors for use in directional cDNA cloning using cohesive ends produced with T4 DNA polymerase.用于使用T4 DNA聚合酶产生的粘性末端进行定向cDNA克隆的功能性克隆载体。

Gene. 1992 Mar 15;112(2):147-55. doi: 10.1016/0378-1119(92)90370-5.

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