一种改进的用于PCR扩增DNA定向亚克隆的无连接酶方法。 - Suppr

An improved ligase-free method for directional subcloning of PCR amplified DNA.

作者信息

Zhou M, Hatahet Z

机构信息

Department of Microbiology and Molecular Genetics, Markey Center for Molecular Genetics, University of Vermont, Burlington 05405, USA.

出版信息

Nucleic Acids Res. 1995 Mar 25;23(6):1089-90. doi: 10.1093/nar/23.6.1089.

DOI:10.1093/nar/23.6.1089

PMID:7731799

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC306811/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4d7a/306811/b387263aeabd/nar00006-0214-a.jpg

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An improved ligase-free method for directional subcloning of PCR amplified DNA.一种改进的用于PCR扩增DNA定向亚克隆的无连接酶方法。

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本文引用的文献

Exonuclease III induced ligase-free directional subcloning of PCR products.核酸外切酶III诱导的PCR产物无连接酶定向亚克隆

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The use of phosphorothioate primers and exonuclease hydrolysis for the preparation of single-stranded PCR products and their detection by solid-phase hybridization.使用硫代磷酸酯引物和核酸外切酶水解制备单链PCR产物及其通过固相杂交进行检测。

PCR Methods Appl. 1994 Apr;3(5):285-91. doi: 10.1101/gr.3.5.285.

Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.用核酸外切酶III进行单向消化可为DNA测序创建靶向断点。

Gene. 1984 Jun;28(3):351-9. doi: 10.1016/0378-1119(84)90153-7.

Gene 6 exonuclease of bacteriophage T7. II. Mechanism of the reaction.噬菌体T7的基因6核酸外切酶。II. 反应机制。

J Biol Chem. 1972 Jan 10;247(1):311-8.

Functional cloning vectors for use in directional cDNA cloning using cohesive ends produced with T4 DNA polymerase.用于使用T4 DNA聚合酶产生的粘性末端进行定向cDNA克隆的功能性克隆载体。

Gene. 1992 Mar 15;112(2):147-55. doi: 10.1016/0378-1119(92)90370-5.

Uracil DNA glycosylase-mediated cloning of polymerase chain reaction-amplified DNA: application to genomic and cDNA cloning.尿嘧啶DNA糖基化酶介导的聚合酶链反应扩增DNA克隆：应用于基因组和cDNA克隆

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Synchronous digestion of SV40 DNA by exonuclease III.用核酸外切酶III对SV40 DNA进行同步消化。

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An improved ligase-free method for directional subcloning of PCR amplified DNA.

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