支气管败血波氏杆菌皮肤坏死毒素（BBD）疫苗从负载BBD的壳聚糖微球中的体外控释。

Controlled release of Bordetella bronchiseptica dermonecrotoxin (BBD) vaccine from BBD-loaded chitosan microspheres in vitro.

作者信息

Jiang Hu-Lin, Park In-Kyu, Shin Na-Ri, Yoo Han-Sang, Akaike Toshihiro, Cho Chong-Su

机构信息

School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea.

出版信息

Arch Pharm Res. 2004 Mar;27(3):346-50. doi: 10.1007/BF02980071.

DOI:10.1007/BF02980071

PMID:15089042

Abstract

Chitosan microspheres were prepared by ionic gelation process with sodium sulfate for nasal vaccine delivery. Bordetella Bronchiseptica Dermonecrotoxin (BBD) as a major virulence factor of a causative agent of atrophic rhinitis (AR) was loaded to the chitosan microspheres for vaccination. Morphology of BBD-loaded chitosan microspheres was observed as spherical shapes. The average particle sizes of the BBD-loaded chitosan microspheres were about 2.69 microm. More BBD was released with an increase of molecular weight of chitosan and with an increase of medium pH in vitro due to weaker intermolecular interaction between chitosan and BBD. Tumor necrosis factor-alpha (TNFalpha) and nitric oxide (NO) from RAW264.7 cells stimulated with BBD-loaded chitosan microspheres were gradually secreted, suggesting that released BBD from chitosan microspheres had immune stimulating activity of AR vaccine.

摘要

通过离子凝胶法用硫酸钠制备壳聚糖微球用于鼻腔疫苗递送。将支气管败血波氏杆菌皮肤坏死毒素（BBD）作为萎缩性鼻炎（AR）病原体的主要毒力因子负载到壳聚糖微球中用于疫苗接种。观察到负载BBD的壳聚糖微球的形态为球形。负载BBD的壳聚糖微球的平均粒径约为2.69微米。由于壳聚糖与BBD之间的分子间相互作用较弱，随着壳聚糖分子量的增加以及体外培养基pH值的升高，更多的BBD被释放出来。用负载BBD的壳聚糖微球刺激的RAW264.7细胞逐渐分泌肿瘤坏死因子-α（TNFα）和一氧化氮（NO），这表明从壳聚糖微球中释放的BBD具有AR疫苗的免疫刺激活性。