壳聚糖微球联合萎缩性鼻炎疫苗免疫刺激活性的体外研究。 [注：原文中“athrophic”拼写错误，应为“atrophic”，译文已修正]
In vitro study of the immune stimulating activity of an atrophic [correction of athrophic] rhinitis vaccine associated to chitosan microspheres.
作者信息
Jiang Hu-Lin, Park In-Kyu, Shin Na-Ri, Kang Sang-Gyun, Yoo Han-Sang, Kim Su-Il, Suh Sang-Bong, Akaike Toshihiro, Cho Chong-Su
机构信息
School of Agricultural Biotechnology, Seoul National University, Seoul, South Korea.
出版信息
Eur J Pharm Biopharm. 2004 Nov;58(3):471-6. doi: 10.1016/j.ejpb.2004.05.006.
Chitosan microspheres (CMs) were prepared by an ionic gelation process with tripolyphosphate and characterized. Bordetella Bronchiseptica Dermonecrotoxin (BBD), a major virulence factor of a causative agent of atrophic rhinitis (AR), was loaded on to the CMs for nasal vaccination. BBD-loaded CMs were observed as aggregated shapes although unloaded CMs were observed as relatively spherical ones. The average particle size of the BBD-loaded CMs was 4.39 microm. The lower the molecular weight of chitosan and the higher the medium pH, the greater was the release of BBD from the BBD-loaded CMs in vitro due to weaker intermolecular interaction between chitosan and BBD. Tumor necrosis factor alpha and nitric oxide from RAW264.7 cells exposed to BBD-loaded CMs were gradually secreted with time, suggesting that released BBD from CMs had immune stimulating activity of AR vaccine in vitro.
壳聚糖微球（CMs）通过与三聚磷酸钠的离子凝胶化过程制备并进行了表征。支气管败血波氏杆菌皮肤坏死毒素（BBD）是萎缩性鼻炎（AR）病原体的一种主要毒力因子，被负载到CMs上用于鼻内疫苗接种。尽管未负载的CMs观察为相对球形，但负载BBD的CMs观察为聚集形状。负载BBD的CMs的平均粒径为4.39微米。壳聚糖分子量越低且介质pH越高，由于壳聚糖与BBD之间的分子间相互作用较弱，负载BBD的CMs在体外释放的BBD就越多。暴露于负载BBD的CMs的RAW264.7细胞分泌的肿瘤坏死因子α和一氧化氮随时间逐渐增加，表明从CMs释放的BBD在体外具有AR疫苗的免疫刺激活性。
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