Mendel F C, Wylegala J A, Fish D R
Department of Anatomical Sciences, State University of New York, Buffalo 14214.
Phys Ther. 1992 Sep;72(9):668-73. doi: 10.1093/ptj/72.9.668.
High voltage pulsed current (HVPC) has been shown to be effective in curbing posttraumatic edema formation in frogs. The purpose of this study was to establish the utility of HVPC in controlling edema formation in a mammalian model. Both feet of 20 anesthetized rats were traumatized following initial determination of hind-limb volumes. Four 30-minute cathodal HVPC treatments at 120 pulses per second and 90% of visible motor threshold interspersed with 30-minute rest periods were applied to one randomly selected hind limb of each rat via the immersion technique. The other hind limb served as a control. Limb volumes were measured after each treatment and rest period. All data were expressed as changes from pretrauma limb volumes in milliliters per kilogram of body weight; these data were analyzed by repeated-measures analyses of variance and post hoc paired t tests. Volumes of untreated limbs were significantly greater than volumes of treated limbs after the second treatment. Evidence of significant treatment effects in frogs, and now rats, provides a compelling rationale for initiating trials of efficacy of cathodal HVPC in inhibiting acute edema formation in humans.
高压脉冲电流（HVPC）已被证明在抑制青蛙创伤后水肿形成方面有效。本研究的目的是确定HVPC在控制哺乳动物模型中水肿形成的效用。在初步测定后肢体积后，对20只麻醉大鼠的双足进行创伤。通过浸泡技术，对每只大鼠随机选择的一个后肢施加每秒120次脉冲、90%可见运动阈值的4次30分钟阴极HVPC治疗，中间穿插30分钟休息期。另一个后肢作为对照。在每次治疗和休息期后测量肢体体积。所有数据均表示为相对于创伤前肢体体积每千克体重的毫升变化量；这些数据通过重复测量方差分析和事后配对t检验进行分析。第二次治疗后，未治疗肢体的体积明显大于治疗肢体的体积。在青蛙以及现在在大鼠中显著治疗效果的证据，为启动阴极HVPC抑制人类急性水肿形成疗效试验提供了令人信服的理由。
