Castigli E, Mancinelli L, Franceschini M, Gianfranceschi G L, Bramucci M, Miano A, Amici D
Institute of Cell Biology, University of Perugia, Italy.
Physiol Chem Phys Med NMR. 1992;24(2):109-17.
A variety of evidence suggests that a family of chromatin peptides (CPs), characterized by 1000D molecular weight, a pH dependent association to DNA and a prevailing presence of acidic amino acids in their structure, is involved in the regulation of genes expression. Nevertheless their action mechanism is still unknown. In our in vitro specific RNA transcription systems the CPs affect the initiation and not the elongation. Furthermore they inhibit the RNA transcription by interaction with the DNA rather than with the enzyme. The phagic in vitro specific RNA transcription is less affected by CPs than the eubacteric system, suggesting a kind of selectivity for target DNA sequences involved in the initiation of transcription.
多种证据表明，一类染色质肽（CPs）参与基因表达的调控。这类肽的分子量为1000D，与DNA的结合呈pH依赖性，且其结构中主要为酸性氨基酸。然而，它们的作用机制仍然未知。在我们的体外特异性RNA转录系统中，CPs影响转录起始而非延伸。此外，它们通过与DNA而非酶相互作用来抑制RNA转录。与真细菌系统相比，吞噬体外特异性RNA转录受CPs的影响较小，这表明对参与转录起始的靶DNA序列具有某种选择性。
