Spena A, Chimenti R, Covello C, De Cicco T, Mazzulla S, Martino G
Department of Cell Biology, University of Calabria, Rende (CS), Italy.
Physiol Chem Phys Med NMR. 1995;27(4):281-91.
A peptide fraction from the mitochondrial DNA of calf's liver was isolated using Drouin's method (1). This peptide fraction, which was extracted at pH 9.5 from an extensively purified mitochondrial DNA (2), has been shown to exert an in vitro regulatory role on the transcription and duplication activity of DNA (3). The same fraction also binds with mitochondrial DNA with a high affinity constant and stabilizes DNA from calf's thymus against thermal denaturation. The peptides from mitochondrial DNA have been subfractionated by fingerprinting-like techniques and one of them has been sequenced.
采用德鲁安方法（1）从小牛肝脏的线粒体DNA中分离出一种肽组分。该肽组分是在pH 9.5条件下从高度纯化的线粒体DNA中提取的（2），已证明其对DNA的转录和复制活性具有体外调节作用（3）。同一组分还以高亲和常数与线粒体DNA结合，并稳定小牛胸腺的DNA以防止热变性。线粒体DNA来源的肽已通过类似指纹图谱的技术进行了细分，其中一种已完成测序。
