Ohashi S, Saku S, Yamamoto K
Department of General & Operative Dentistry, School of Dentistry, Asahi University, Japan.
J Oral Rehabil. 2004 Apr;31(4):364-7. doi: 10.1111/j.1365-2842.2004.01200.x.
YDA filler is an antibacterial agent that is currently in commercial dental use. In this study, we attempted to determine whether it exerts an antibacterial effect on human saliva bacteria, and to determine whether it can be used in dental materials. CFUs in 1 mL stimulated human saliva were examined using blood agar and mitis salivarius agar after immersion, with or without YDA filler. The antibacterial effect was compared with that of Ketac-Silver. Dental materials containing 5% wt YDA filler were prepared for in vitro testing on S. mutans and A. viscosus. Furthermore, we examined the in vitro cytotoxicity of experimental MMA resin containing YDA filler on HeLa cells. Human saliva bacteria and mutans streptococci showed reduced viability following exposure to YDA filler after 12 h. The concentration of silver ions released by YDA filler was below 1 ppm after 12 h. Two tested strains showed reduced viability following exposure to dental materials containing YDA filler. In another experiment, MMA resin containing YDA filler did not show cytotoxicity on HeLa cells after 24- and 48-h exposure. Thus, YDA filler may help in the development of antibacterial dental materials, such as composite resin, glass-ionomer or temporary cement.
YDA填料是一种目前在牙科商业应用中的抗菌剂。在本研究中，我们试图确定它是否对人类唾液细菌产生抗菌作用，以及它是否可用于牙科材料。在有或没有YDA填料浸泡后，使用血琼脂和唾液变形链球菌琼脂检测1 mL刺激的人类唾液中的菌落形成单位（CFU）。将抗菌效果与Ketac - Silver的抗菌效果进行比较。制备了含有5%重量YDA填料的牙科材料用于对变形链球菌和粘性放线菌的体外测试。此外，我们检测了含有YDA填料的实验性甲基丙烯酸甲酯（MMA）树脂对HeLa细胞的体外细胞毒性。12小时后，人类唾液细菌和变形链球菌在接触YDA填料后活力降低。12小时后，YDA填料释放的银离子浓度低于1 ppm。两种受试菌株在接触含有YDA填料的牙科材料后活力降低。在另一项实验中，含有YDA填料的MMA树脂在暴露24小时和48小时后对HeLa细胞未显示出细胞毒性。因此，YDA填料可能有助于开发抗菌牙科材料，如复合树脂、玻璃离子水门汀或临时粘结剂。
