Rajeshkumar S, Malarkodi C
Department of Biochemistry, Adhiparasakthi College of Arts and Science, Kalavai, Vellore District 632506, Tamil Nadu, India.
Environmental Nanotechnology Division, Sri Paramakalyani Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi 627 412, Tamil Nadu, India.
Bioinorg Chem Appl. 2014;2014:581890. doi: 10.1155/2014/581890. Epub 2014 Sep 17.
Biosynthesis of silver nanoparticles using Planomicrobium sp. and to explore the antibacterial activity against food borne pathogenic bacteria Bacillus subtilis, (3053) Klebsiella planticola (2727) Klebsiella pneumoniae (MAA) Serratia nematodiphila (CAA) and Escherichia coli. In the current studies, 1 mM of silver nitrate was added into 100 mL of Planomicrobium sp. culture supernatant. The bioreduction of pure AgNO3 was characterized by UV-visible spectroscopy, X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), energy-dispersive analysis (EDS), transmission electron microscopy (TEM), and Fourier transform infrared (FT-IR) analysis. The formation of silver nanoparticles was confirmed by the presence of an absorption peak at 400 nm using UV-visible spectrophotometry. The morphology and size of the silver nanoparticles was monitored by TEM and SEM. Crystal structure was obtained by carrying out X-ray diffraction studies and it showed face centered cubic (FCC) structure. The bactericidal effect of silver nanoparticles was compared based on diameter of inhibition zone in well method. Bacterial sensitivity to nanoparticles a key factor in manufacture the suitable for long life application in food packaging and food safety. Food safety is a worldwide health goal and the food borne diseases get a main disaster on health. Therefore, controlling of bacterial pathogens in food is credit of harms associated to health and safety.
利用扁平微球菌合成银纳米颗粒，并探究其对食源致病菌枯草芽孢杆菌（3053）、植生克雷伯菌（2727）、肺炎克雷伯菌（MAA）、嗜线虫沙雷氏菌（CAA）和大肠杆菌的抗菌活性。在当前研究中，将1 mM硝酸银添加到100 mL扁平微球菌培养上清液中。通过紫外可见光谱、X射线衍射分析（XRD）、扫描电子显微镜（SEM）、能量色散分析（EDS）、透射电子显微镜（TEM）和傅里叶变换红外（FT-IR）分析对纯硝酸银的生物还原进行了表征。使用紫外可见分光光度法在400 nm处出现吸收峰证实了银纳米颗粒的形成。通过TEM和SEM监测银纳米颗粒的形态和尺寸。通过进行X射线衍射研究获得晶体结构，结果显示为面心立方（FCC）结构。基于孔穴法中的抑菌圈直径比较了银纳米颗粒的杀菌效果。细菌对纳米颗粒的敏感性是制造适用于食品包装和食品安全的长寿命产品的关键因素。食品安全是一个全球性的健康目标，食源性疾病是对健康的主要危害。因此，控制食品中的细菌病原体是关乎健康和安全相关危害的重要任务。
