Koblavi-Dème Stéphania, Kestens Luc, Hanson Debra, Otten Ronald A, Borget Marie-Yolande, Bilé Célestin, Wiktor Stefan Z, Roels Thierry H, Chorba Terence, Nkengasong John N
Projet RETRO-CI, Abidjan, Côte d'Ivoire, the Institute of Tropical Medicine, Antwerp, Belgium.
AIDS. 2004 Feb 20;18(3):413-9. doi: 10.1097/00002030-200402200-00006.
To determine whether blood plasma levels of HIV-2 RNA viral loads and immune activation markers differ between persons infected with HIV-2 only and those dually infected with HIV-1 and HIV-2.
Between September 1996 and February 2000, we collected, analyzed and compared levels of HIV-2 RNA in plasma and immune activation markers among 52 persons infected with HIV-2 alone and 75 with confirmed dual infection. We also compared viral load and immune activation in patients who were infected with HIV-1 only and those who were dually infected.
When we conducted a CD4 T-cell count-stratified multivariate analysis of HIV-2 viral load, controlling for difference in CD4 T-cell counts, age and sex: at < 200 x 10 CD4 T cells/l, HIV-2 viral load was 2.0 log10 copies/ml lower in dually infected patients than in HIV-2 only patients (P < 0.0001). At CD4 T-cell counts between 200 x 10 and 500 x 10/l, HIV-2 viral load was 0.3 log10 copies/ml lower in dually infected patients (P = 0.45). However, at CD4 T-cells counts > 500 x 10/l, HIV-2 viral load was 0.9 log10 copies/ml higher in dually infected patients (P < 0.0001). Dually infected persons with undetectable HIV-2 viral loads had significantly higher median levels of CD8 T cells expressing CD38 (P < 0.001) and HLA-DR (P = 0.01) than HIV-2 only infected patients.
These results suggest that in dual infection, the level of HIV-2 replication depends on the immune status of the patients, with HIV-1 out-replicating HIV-2 as disease progress.
确定仅感染HIV-2的人与同时感染HIV-1和HIV-2的人之间，血浆中HIV-2 RNA病毒载量和免疫激活标志物水平是否存在差异。
在1996年9月至2000年2月期间，我们收集、分析并比较了52名单独感染HIV-2的人和75名确诊为双重感染的人的血浆中HIV-2 RNA水平和免疫激活标志物。我们还比较了仅感染HIV-1的患者和双重感染患者的病毒载量及免疫激活情况。
当我们对HIV-2病毒载量进行CD4 T细胞计数分层多变量分析时，控制CD4 T细胞计数、年龄和性别的差异：在CD4 T细胞计数<200×10⁶个/升时，双重感染患者的HIV-2病毒载量比仅感染HIV-2的患者低2.0 log₁₀拷贝/毫升（P<0.0001）。在CD4 T细胞计数介于200×10⁶和500×10⁶个/升之间时，双重感染患者的HIV-2病毒载量低0.3 log₁₀拷贝/毫升（P = 0.45）。然而，在CD4 T细胞计数>500×10⁶个/升时，双重感染患者的HIV-2病毒载量高0.9 log₁₀拷贝/毫升（P<0.0001）。HIV-2病毒载量检测不到的双重感染患者，其表达CD38的CD8 T细胞（P<0.001）和HLA-DR（P = 0.01）的中位数水平显著高于仅感染HIV-2的患者。
这些结果表明，在双重感染中，HIV-2复制水平取决于患者的免疫状态，随着疾病进展，HIV-1的复制超过HIV-2。
