Haginaka Jun, Kagawa Chino
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, 11-68 Koshien Kyuban-cho, Nishinomiya, Hyogo 663-8179, Japan.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 May 5;804(1):19-24. doi: 10.1016/j.jchromb.2003.12.037.
Uniformly sized molecularly imprinted polymers (MIPs) for d-chlorpheniramine (CP) and -brompheniramine (BP) have been prepared by a multi-step swelling and polymerization method using methacrylic acid (MAA) or 2-(trifluoromethyl)acrylic acid (TFMAA) and ethylene glycol dimethacrylate (EDMA) as a functional monomer and cross-linker, respectively. The retentive and enantioselective properties of CP, BP and their structurally related compounds on the MIPs were evaluated using hydro-organic mobile phases. CP and BP enantiomers were retained the most as a monovalent cation on MAA-co-EDMA polymers and a divalent cation on TFMAA-co-EDMA polymers. Ion exchange and hydrophobic interactions could mainly work for the retention and enantioseparation of CP and BP on both MAA-co-EDMA and TFMAA-co-EDMA polymers in hydro-organic mobile phases. Though the respective MIPs gave the highest enantioselectivity for the template molecule, cross-reactivity for CP and BP was observed with them.
通过多步溶胀聚合方法，分别以甲基丙烯酸（MAA）或2-（三氟甲基）丙烯酸（TFMAA）为功能单体、乙二醇二甲基丙烯酸酯（EDMA）为交联剂，制备了尺寸均匀的用于右氯苯那敏（CP）和溴苯那敏（BP）的分子印迹聚合物（MIP）。使用水-有机流动相评估了CP、BP及其结构相关化合物在MIP上的保留和对映选择性。CP和BP对映体在MAA-co-EDMA聚合物上作为单价阳离子、在TFMAA-co-EDMA聚合物上作为二价阳离子保留最多。在水-有机流动相中，离子交换和疏水相互作用主要作用于CP和BP在MAA-co-EDMA和TFMAA-co-EDMA聚合物上的保留和对映体分离。尽管各自的MIP对模板分子具有最高的对映选择性，但观察到它们对CP和BP有交叉反应性。
