Sambe Haruyo, Hoshina Kaori, Moaddel Ruin, Wainer Irving W, Haginaka Jun
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, 11-68 Koshien Kyuban-cho, Nishinomiya 663-8179, Japan.
J Chromatogr A. 2006 Nov 17;1134(1-2):88-94. doi: 10.1016/j.chroma.2006.08.073. Epub 2006 Sep 15.
Uniformly-sized, molecularly imprinted polymers (MIPs) for (S)-nicotine have been prepared by a precipitation polymerization method using methacrylic acid (MAA) or 2-(trifluoromethyl)acrylic acid (TFMAA) as a functional monomer and divinylbenzene (DVB) as a cross-linker in a mixture of toluene and acetonitrile. The (S)-nicotine-imprinted MAA-co-DVB polymers were monodispersed microspheres of about 4 microm in diameter, while the TFMAA-co-DVB ones were gel-like. Molecular recognition abilities of the former MIPs were evaluated for nicotine and its structurally related compounds in liquid chromatography using a mixture of sodium phosphate buffer and acetonitrile as the eluent. Enantioseparation of nicotine was attained using the (S)-nicotine-imprinted MAA-co-DVB polymers. Furthermore, they could selectively trap nicotine in cigarette smoke extracts.
已采用沉淀聚合法，以甲基丙烯酸（MAA）或2-（三氟甲基）丙烯酸（TFMAA）作为功能单体、二乙烯基苯（DVB）作为交联剂，在甲苯和乙腈的混合物中制备了用于（S）-尼古丁的尺寸均匀的分子印迹聚合物（MIP）。（S）-尼古丁印迹的MAA-co-DVB聚合物是直径约4微米的单分散微球，而TFMAA-co-DVB聚合物则呈凝胶状。使用磷酸钠缓冲液和乙腈的混合物作为洗脱剂，在液相色谱中评估了前一种MIP对尼古丁及其结构相关化合物的分子识别能力。使用（S）-尼古丁印迹的MAA-co-DVB聚合物实现了尼古丁的对映体分离。此外，它们可以选择性地捕获香烟烟雾提取物中的尼古丁。
