Pharmaceutical and Molecular Biotechnology Research Centre, Waterford Institute of Technology, Waterford, Ireland.
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Nov 15;879(30):3523-30. doi: 10.1016/j.jchromb.2011.09.036. Epub 2011 Sep 23.
A novel non-stabilised aqueous suspension polymerisation methodology for the preparation of spherical molecularly imprinted polymers is described with chlorpheniramine (CP), d-chlorpheniramine (d-CP), brompheniramine (BP) and d-brompheniramine (d-BP) as the templates, respectively. Using this rapid and simple technique, controlled polymer beads in the low micron range with narrow size distributions were generated by photo-polymerisation. The use of agitation speed as a method of controlling bead size distribution was demonstrated. Enantioselective properties of the imprinted beads were examined and the polymers prepared using d-chlorpheniramine and d-brompheniramine were capable of discriminating between the enantiomers of the template. Cross-selectivity studies were performed by batch rebinding with the influence of template size and functional group orientation of analytes on the recognition properties of the imprinted polymers investigated. Physical characteristics of all polymers were studied by nitrogen sorption porosimetry, particle size analysis and scanning electron microscopy (SEM) in order to gain an insight into the role of such properties on retention behaviour.
一种新颖的非稳定水相悬浮聚合方法被用于制备球形分子印迹聚合物，分别以氯苯那敏（CP）、d-氯苯那敏（d-CP）、溴苯那敏（BP）和 d-溴苯那敏（d-BP）作为模板。使用这种快速简单的技术，通过光聚合生成了具有低微米范围和窄粒径分布的受控聚合物珠。证明了搅拌速度可作为控制珠粒径分布的方法。对印迹珠的对映选择性进行了研究，使用 d-氯苯那敏和 d-溴苯那敏制备的聚合物能够区分模板的对映体。通过分批再结合研究了交叉选择性，考察了模板大小和分析物官能团取向对印迹聚合物识别性能的影响。通过氮气吸附孔隙率、粒度分析和扫描电子显微镜（SEM）研究了所有聚合物的物理特性，以便深入了解这些特性对保留行为的作用。
