Wang Hong Ying, Xia Shao Ling, Sun Hong, Liu Yu Kun, Cao Shao Kui, Kobayashi Takaomi
Material Engineering School of Zhengzhou University, Zhengzhou 450052, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 May 5;804(1):127-34. doi: 10.1016/j.jchromb.2004.01.036.
Uracil (URA) was selected as a template for preparing molecularly imprinted membranes of poly(acrylonitrile-co-methylacrylic acid) [P(AN-co-MAA)] using the phase inversion technique. This study used Fourier transform infra-red (FT-IR) and (1)H nuclear magnetic resonance (NMR) spectroscopic studies to characterize the polymer-template interaction and scanning electron microscopy (SEM) and atomic force microscopy (AFM) for morphology of the URA imprinted membrane. Resultant membranes had typical ultrafiltration structure with porous morphology and showed a permeation flux of 3.5 x 10 9-5)m(3)/(m(2)s) for 32 microM URA aqueous solution. Permselective binding to the target molecule was observed in permeation experiments with 7.9 micromol/g binding capacity of URA. Binding selectivity was discussed for URA and its analogs, dimethyluracil (DMURA) and caffeine (CAF), with 0.6 and 0.8 micromol/g binding capacity, respectively.
选用尿嘧啶（URA）作为模板，采用相转化技术制备聚（丙烯腈 - 共 - 甲基丙烯酸）[P（AN - 共 - MAA）]分子印迹膜。本研究利用傅里叶变换红外光谱（FT - IR）和氢核磁共振（NMR）光谱研究来表征聚合物与模板之间的相互作用，并通过扫描电子显微镜（SEM）和原子力显微镜（AFM）观察URA印迹膜的形态。所得膜具有典型的超滤结构，呈多孔形态，对于32 microM的URA水溶液，其渗透通量为3.5×10⁻⁵ m³/（m²·s）。在渗透实验中观察到对目标分子的渗透选择性结合，URA的结合容量为7.9微摩尔/克。还讨论了URA及其类似物二甲基尿嘧啶（DMURA）和咖啡因（CAF）的结合选择性，它们的结合容量分别为0.6和0.8微摩尔/克。
