Fernández-González A, Badía Laíño R, Diaz-García M E, Guardia L, Viale A
Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, C/ Julian Claveria 8, Oviedo 33006, Spain.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 May 5;804(1):247-54. doi: 10.1016/j.jchromb.2003.12.030.
Sol-gel imprinted materials were prepared against nafcillin, a semisynthetic beta-lactamic antibiotic employed in the treatment of serious infections caused by penicillinase-producing staphylococci. Two approaches were addressed for preparation of the imprinted materials and the controls: as conventional monoliths, which were ground and sieved to a desired particle size for rebinding analysis, and as films on supporting glass slides. The specific binding sites that are created during the imprinting process are analyzed via selective room temperature phosphorescence (RTP) (sol-gel films) measurements as well as via competitive room temperature phosphorescence ligand assay. Results demonstrated the importance of the physical configuration of the imprinted material for minimizing non-specific binding. The close similarities between the structures of different beta-lactamic antibiotics made it possible to interpret the roles of the template structure on specific molecular recognition. In this article, we introduce the use of room temperature phosphorescence as selective transduction method for the template. The imprinted sol-gel films displayed enhanced specific binding characteristics respect to the monolithic sol-gel and can be envisaged for the use as recognition matrices for the screening and rapid selection of antibiotics from a combinatorial library or for the rapid control of nafcillin in biological samples (e.g. milk, serum, urine).
针对萘夫西林制备了溶胶 - 凝胶印迹材料，萘夫西林是一种半合成β - 内酰胺类抗生素，用于治疗由产青霉素酶葡萄球菌引起的严重感染。制备印迹材料和对照物采用了两种方法：一种是制备常规整体柱，将其研磨并筛分至所需粒径用于再结合分析；另一种是在支撑载玻片上制备薄膜。通过选择性室温磷光（RTP）（溶胶 - 凝胶薄膜）测量以及竞争性室温磷光配体测定法，对印迹过程中产生的特异性结合位点进行分析。结果表明印迹材料的物理构型对于最小化非特异性结合的重要性。不同β - 内酰胺类抗生素结构之间的紧密相似性使得能够解释模板结构在特异性分子识别中的作用。在本文中，我们介绍了使用室温磷光作为模板的选择性传感方法。印迹溶胶 - 凝胶薄膜相对于整体溶胶 - 凝胶表现出增强的特异性结合特性，可设想用作识别基质，用于从组合文库中筛选和快速选择抗生素，或用于生物样品（如牛奶、血清、尿液）中萘夫西林的快速检测。
