Zhang Zhaohui, Long Yumei, Nie Lihua, Yao Shouzhuo
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Biosens Bioelectron. 2006 Jan 15;21(7):1244-51. doi: 10.1016/j.bios.2005.05.009. Epub 2005 Jun 23.
A novel method of combining sol-gel and self-assembly technology to prepare a human serum albumin (HSA)-imprinted film on the surface of piezoelectric quartz crystal (PQC) Au-electrode modified with thioglycolic acid was described in this paper. The imprinting process was characterized by using the piezoelectric quartz crystal impedance (PQCI) technique and electrochemical impedance technique. Scanning electron microscope (SEM) was employed to characterize the surface morphology of the resultant imprinted film. The piezoelectric technique and electrochemical impedance technique were also employed to investigate the binding performance of the sol-gel-imprinted film with the template protein. The results showed that the imprinted PQC film can give selective recognition to the template protein. The effects of salts and solvents on the binding capacity of the imprinted film with protein were discussed in detail. Other influencing factors (temperature and pH) have also been investigated. This self-assembly sol-gel imprinting technique was proved to be an alternative method for the preparation of biomacromolecule-imprinted thin film.
本文描述了一种将溶胶-凝胶法与自组装技术相结合的新方法，用于在经巯基乙酸修饰的压电石英晶体（PQC）金电极表面制备人血清白蛋白（HSA）印迹膜。通过压电石英晶体阻抗（PQCI）技术和电化学阻抗技术对印迹过程进行表征。利用扫描电子显微镜（SEM）对所得印迹膜的表面形貌进行表征。还采用压电技术和电化学阻抗技术研究溶胶-凝胶印迹膜与模板蛋白的结合性能。结果表明，印迹PQC膜能够对模板蛋白进行选择性识别。详细讨论了盐和溶剂对印迹膜与蛋白质结合能力的影响。还研究了其他影响因素（温度和pH值）。这种自组装溶胶-凝胶印迹技术被证明是制备生物大分子印迹薄膜的一种替代方法。
