Guardia Laura, Badía Rosana, Díaz-García Marta E
Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Av. Julián Clavería 8, 33006-Oviedo, Spain.
J Agric Food Chem. 2007 Feb 7;55(3):566-70. doi: 10.1021/jf062058o.
A study has been made of the analytical application of a nafcillin-imprinted sol-gel to the direct determination of the beta-lactamic antibiotic in spiked milk-based samples using a room temperature phosphorescent flow-through system. The influence of the sample matrix on the transduction and the recognition processes was statistically determined, and results demonstrated that the imprinted sol-gel optosensing system could be effectively applied to real sample analysis. The analytical performance characteristics were as follows: The detection limit results for aqueous and skimmed milk were 5.8 x 10(-6) and 3.3 x 10(-5) mol L(-1), respectively, and a relative standard deviation less than 5% was found for both matrices. Statistical analysis of variance studies have been shown to have no significant effect on different skimmed milk commercial products over the imprinted material recognition. This fact provides an indicator of the ruggedness/robustness of the proposed analytical system and the possibility to use external real matrix calibration. Application of the method to nafcillin analysis in other milk-based samples is outlined.
一项关于萘夫西林印迹溶胶 - 凝胶在使用室温磷光流通系统直接测定加标乳基样品中β - 内酰胺类抗生素的分析应用研究。通过统计确定了样品基质对传感和识别过程的影响，结果表明印迹溶胶 - 凝胶光传感系统可有效应用于实际样品分析。分析性能特征如下：水性样品和脱脂乳的检测限结果分别为5.8×10⁻⁶和3.3×10⁻⁵ mol L⁻¹，两种基质的相对标准偏差均小于5%。方差统计分析研究表明，不同的脱脂乳商业产品对印迹材料识别没有显著影响。这一事实表明了所提出分析系统的耐用性/稳健性以及使用外部实际基质校准的可能性。概述了该方法在其他乳基样品中萘夫西林分析的应用。
