Padmakumar V C, Abraham Sabu, Braune Stephan, Noegel Angelika A, Tunggal Budi, Karakesisoglou Iakowos, Korenbaum Elena
Center for Biochemistry, Institute of Biochemistry I, Medical Faculty, University of Cologne, 50931 Cologne, FRG Germany.
Exp Cell Res. 2004 May 1;295(2):330-9. doi: 10.1016/j.yexcr.2004.01.014.
Enaptin belongs to a family of recently identified giant proteins that associate with the F-actin cytoskeleton as well as the nuclear membrane. It is composed of an N-terminal alpha-actinin type actin-binding domain (ABD) followed by a long coiled coil rod and a transmembrane domain at the C-terminus. The ABD binds to F-actin in vivo and in vitro and leads to bundle formation. The human Enaptin gene spreads over 515 kb and gives rise to several splicing isoforms (Nesprin-1, Myne-1, Syne-1, CPG2). The longest assembled cDNA encompasses 27,669 bp and predicts a 1014 kDa protein. Antibodies against the ABD of Enaptin localise the protein at F-actin-rich structures throughout the cell and in focal contacts as well as at the nuclear envelope. In COS7 cells, the protein is also present within the nuclear compartment. With the discovery of the actin-binding properties of Enaptin and the highly homologous Nuance, we define a family of proteins that integrate the cytoskeleton with the nucleoskeleton.
Enaptin属于最近发现的一类巨型蛋白质家族，它与F-肌动蛋白细胞骨架以及核膜相关联。它由一个N端的α-辅肌动蛋白型肌动蛋白结合结构域（ABD）、一个长的卷曲螺旋杆以及C端的一个跨膜结构域组成。ABD在体内和体外都能与F-肌动蛋白结合并导致肌动蛋白束的形成。人类Enaptin基因跨度超过515 kb，产生多种剪接异构体（Nesprin-1、Myne-1、Syne-1、CPG2）。最长的组装cDNA包含27,669 bp，预测编码一个1014 kDa的蛋白质。针对Enaptin的ABD的抗体将该蛋白定位在整个细胞中富含F-肌动蛋白的结构、粘着斑以及核膜处。在COS7细胞中，该蛋白也存在于核区室中。随着Enaptin以及高度同源的Nuance的肌动蛋白结合特性的发现，我们定义了一类将细胞骨架与核骨架整合在一起的蛋白质家族。
