澳大利亚淡水小龙虾（Cherax destructor）（甲壳纲：十足目：拟螯虾科）的线粒体DNA全序列：揭示一种新的基因排列顺序
Complete mitochondrial DNA sequence of the Australian freshwater crayfish, Cherax destructor (Crustacea: Decapoda: Parastacidae): a novel gene order revealed.
作者信息
Miller Adam D, Nguyen Thuy T T, Burridge Christopher P, Austin Christopher M
机构信息
School of Ecology and Environment, Deakin University, P.O. Box 423, Warrnambool, Victoria 3280, Australia.
出版信息
Gene. 2004 Apr 28;331:65-72. doi: 10.1016/j.gene.2004.01.022.
The complete mitochondrial DNA sequence was determined for the Australian freshwater crayfish Cherax destructor (Crustacea: Decapoda: Parastacidae). The 15,895-bp genome is circular with the same gene composition as that found in other metazoans. However, we report a novel gene arrangement with respect to the putative arthropod ancestral gene order and all other arthropod mitochondrial genomes sequenced to date. It is apparent that 11 genes have been translocated (ND1, ND4, ND4L, Cyt b, srRNA, and tRNAs Ser(UGA), Leu(CUN), Ile, Cys, Pro, and Val), two of which have also undergone inversions (tRNAs Pro and Val). The 'duplication/random loss' mechanism is a plausible model for the observed translocations, while 'intramitochondrial recombination' may account for the gene inversions. In addition, the arrangement of rRNA genes is incompatible with current mitochondrial transcription models, and suggests that a different transcription mechanism may operate in C. destructor.
已测定澳大利亚淡水小龙虾克氏原螯虾（甲壳纲：十足目：拟螯虾科）的完整线粒体DNA序列。该15,895碱基对的基因组呈环状，其基因组成与其他后生动物相同。然而，相对于假定的节肢动物祖先基因顺序以及迄今测序的所有其他节肢动物线粒体基因组，我们报告了一种新的基因排列。显然有11个基因发生了易位（ND1、ND4、ND4L、Cyt b、srRNA以及tRNA Ser(UGA)、Leu(CUN)、Ile、Cys、Pro和Val），其中两个基因还发生了倒位（tRNA Pro和Val）。“复制/随机丢失”机制是所观察到的易位的一个合理模型，而“线粒体内重组”可能是基因倒位的原因。此外，rRNA基因的排列与当前的线粒体转录模型不相符，这表明在克氏原螯虾中可能存在不同的转录机制。
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