Chatterjee Nando K, Moore Dennison W, Monroe Stephan S, Glass Roger I, Cambridge Michael J, Kondracki Stan F, Morse Dale L
New York State Department of Health, Slingerlands, NY 12159, USA.
Clin Infect Dis. 2004 Apr 15;38 Suppl 3:S303-10. doi: 10.1086/381600.
This investigation evaluated the role of Norwalk-like virus (NLV) and other viruses (rotavirus, enteric adenovirus, and enterovirus) in 11 outbreaks of acute nonbacterial gastroenteritis that occurred in multiple settings in a span of 18 months in New York State. To determine the etiology of illness, patients' stool specimens were analyzed with a combination of reverse-transcription polymerase chain reaction (RT-PCR) and nucleotide sequencing, cell culture, and ELISA diagnostic techniques. NLV was detected from all of these outbreaks, with an overall detection rate of 64% (51 of 79) for all specimens tested. Repeated attempts to isolate other viral pathogens were unsuccessful. Phylogenetic analysis of a subset of 27 specimens from these outbreaks showed the presence of both genogroup I and genogroup II NLVs. A spectrum of different nucleotide sequences were detected, demonstrating interoutbreak sequence variation and unrelated infections. NLV is a significant causative agent of diarrhea outbreaks in New York State.
本调查评估了诺沃克样病毒（NLV）及其他病毒（轮状病毒、肠道腺病毒和肠道病毒）在纽约州18个月内多个场所发生的11起急性非细菌性胃肠炎暴发中的作用。为确定病因，采用逆转录聚合酶链反应（RT-PCR）与核苷酸测序、细胞培养及ELISA诊断技术相结合的方法对患者粪便标本进行分析。所有这些暴发中均检测到NLV，所有检测标本的总体检出率为64%（79份标本中的51份）。多次尝试分离其他病毒病原体均未成功。对这些暴发中的27份标本进行的系统发育分析显示，同时存在基因群I和基因群II的NLV。检测到一系列不同的核苷酸序列，表明暴发间存在序列变异且感染无相关性。NLV是纽约州腹泻暴发的重要病原体。
