Larose M, St-Amand J, Yoshioka M, Belleau P, Morissette J, Labrie C, Raymond V, Labrie F
Molecular Endocrinology and Oncology Research Center, Department of Anatomy and Physiology, Laval University Medical Center, 2705 Boulevard Laurier-BlocT, Quebec G1V 4G2, Canada.
Mol Reprod Dev. 2004 Jun;68(2):142-8. doi: 10.1002/mrd.20065.
The aim of this study was to identify the transcriptome of the normal mouse uterus by Serial Analysis of Gene Expression method. mRNA was extracted from the uterus and also from the gastrocnemius muscle of mice. Short sequences (tags), each one usually corresponding to a distinct transcript, were isolated and concatemerized into long DNA molecules which were cloned and sequenced. We detected 44,484 tags for the uterus and 42,518 tags for the muscle, representing 14,543 and 14,958 potential transcript species, respectively. Seventy-five and sixty-nine genes were expressed at more than 0.1%, thus corresponding to 37 and 34% of the mRNA population detected in the respective tissues. In both cases, the most highly expressed genes are especially involved in muscle contraction, energy metabolism, and protein synthesis. Compared to skeletal muscle, some differentially expressed genes in the uterus are likely to correspond to its specific reproductive functions. The majority of these genes remain to be characterized. More than 70% of the different tags detected in the uterus did not match any sequence in the public databases and can represent novel or poorly identified genes. This study is the first quantitative description of the transcriptome of the uterus.
本研究的目的是通过基因表达序列分析方法鉴定正常小鼠子宫的转录组。从小鼠子宫以及腓肠肌中提取mRNA。分离出短序列（标签），每个标签通常对应一个独特的转录本，然后将这些标签连接成较长的DNA分子，进行克隆和测序。我们检测到子宫有44,484个标签，肌肉有42,518个标签，分别代表14,543和14,958种潜在转录本。分别有75个和69个基因表达水平超过0.1%，分别占各自组织中检测到的mRNA群体的37%和34%。在这两种情况下，表达水平最高的基因尤其参与肌肉收缩、能量代谢和蛋白质合成。与骨骼肌相比，子宫中一些差异表达的基因可能与其特定的生殖功能相对应。这些基因中的大多数仍有待表征。在子宫中检测到的不同标签中，超过70%与公共数据库中的任何序列都不匹配，可能代表新的或鉴定不佳的基因。本研究是对子宫转录组的首次定量描述。
