Bolduc Carl, Larose Marianne, Lafond Nicolas, Yoshioka Mayumi, Rodrigue Marc-André, Morissette Jean, Labrie Claude, Raymond Vincent, St-Amand Jonny
Molecular Endocrinology and Oncology Research Center, Laval Univerisity Medical Center and Laval University, Quebec, Canada.
Obes Res. 2004 May;12(5):750-7. doi: 10.1038/oby.2004.90.
To describe the genomic expression profile or transcriptome of adipose tissue using the serial analysis of gene expression method.
The serial analysis of gene expression strategy is based on isolation of short sequences (tags), which usually correspond to unique transcripts, and on their concatenation into long DNA molecules, which are then cloned and sequenced. Experiments were performed with mRNA from retroperitoneal adipose tissue of male C57BL6 mice.
We isolated 45,996 tags corresponding to more than 17,000 different genes. Eighty-eight genes were expressed at more than 0.1% of the total population and represented 26% of the mRNA population identified. The most expressed genes were: carbonic anhydrase 3 (1.97%), cytochrome c oxidase (COX) 1 (1.47%), COX2 (1.25%), diazepam binding inhibitor (1.04%), a novel transcript (0.87%), COX3 (0.55%), fatty acid-binding protein 4 (0.55%), and NADH dehydrogenase 4 (0.52%). Other genes known to be expressed in adipose tissue, such as uncoupling protein 2, angiotensinogen, adipsin, and insulin-like growth factor 1, were found at a lower level. Several tags corresponding to novel transcripts were also found.
To our knowledge, the present results provide for the first time a quantitative description of the transcriptome in adipose tissue.
使用基因表达序列分析方法描述脂肪组织的基因组表达谱或转录组。
基因表达序列分析策略基于短序列（标签）的分离，这些标签通常对应于独特的转录本，并基于它们连接成长DNA分子，然后进行克隆和测序。实验使用雄性C57BL6小鼠腹膜后脂肪组织的mRNA进行。
我们分离出了45,996个标签，对应于超过17,000个不同的基因。88个基因的表达量占总群体的0.1%以上，占已鉴定mRNA群体的26%。表达量最高的基因有：碳酸酐酶3（1.97%）、细胞色素c氧化酶（COX）1（1.47%）、COX2（1.25%）、地西泮结合抑制剂（1.04%）、一个新转录本（0.87%）、COX3（0.55%）、脂肪酸结合蛋白4（0.55%）和NADH脱氢酶4（0.52%）。其他已知在脂肪组织中表达的基因，如解偶联蛋白2、血管紧张素原、脂肪酶和胰岛素样生长因子1，其表达水平较低。还发现了几个对应于新转录本的标签。
据我们所知，目前的结果首次提供了脂肪组织转录组的定量描述。
