Viaggiu Emanuela, Melchiorre Serena, Volpi Fabrizio, Di Corcia Antonio, Mancini Roberta, Garibaldi Letizia, Crichigno Giuseppe, Bruno Milena
Department of Environmental Hygiene, National Health Institute, Viale Regina Elena 299, 00161 Rome, Italy.
Environ Toxicol. 2004 Jun;19(3):191-7. doi: 10.1002/tox.20011.
A heavy algal bloom occurring in a fishing pond in northern Italy full of Salmo trutta was examined for algae taxonomy and toxic production. The dominant algal species (98%) was identified as the cyanobacterium Planktothrix rubescens (D.C. ex GOMONT) Komarek Anagnostidis, based on morphological examination, and it was revealed to be toxic in mouse and Vibrio fischeri bioassays. The toxin was identified as anatoxin-a using high-performance liquid chromatography and confirmed using liquid chromatography-mass spectrometry (LC-MS). The mouse bioassay gave signs of poisoning, as previously reported for anatoxin-a. The LC-MS confirmed the presence of an anatoxin-a peak at m/z 166 (M+H+). The content of toxin in the field population was estimated at 12.13 microg/g of fresh cells. The bloom was sustained by the very high N/P ratio in the water. This is the first report in Italy of an anatoxin-a-producing Planktothrix rubescens population.
对意大利北部一个满是虹鳟鱼的鱼塘中出现的严重藻华进行了藻类分类学和毒素产生情况的检测。基于形态学检查，优势藻类物种（98%）被鉴定为蓝藻门的微红颤藻（Planktothrix rubescens (D.C. ex GOMONT) Komarek Anagnostidis），并且在小鼠和费氏弧菌生物测定中显示具有毒性。使用高效液相色谱法将毒素鉴定为anatoxin-a，并通过液相色谱-质谱联用（LC-MS）进行了确认。小鼠生物测定出现了中毒迹象，这与之前报道的anatoxin-a情况一致。LC-MS证实了在m/z 166（M+H+）处存在anatoxin-a峰。野外群体中的毒素含量估计为每克新鲜细胞12.13微克。水体中极高的氮磷比维持了藻华的持续。这是意大利关于产生anatoxin-a的微红颤藻群体的首次报道。
