Mahmood N A, Carmichael W W, Pfahler D
Department of Biological Sciences, Wright State University, Dayton, OH 45435.
Am J Vet Res. 1988 Apr;49(4):500-3.
Cyanobacteria (blue-green algae) implicated in the deaths of 9 dogs at Richmond Lake, SD, on Aug 26, 1985, were analyzed. The dominant cyanobacterial species from the water sample was Anabaena flos-aquae. The lyophilized bloom material or the high-performance liquid chromatography purified toxin peak, when administered to mice IP, induced clinical signs of salivation, lacrimation, urinary incontinence, defecation, convulsion, fasciculation, and respiratory arrest. Further comparison of the semipurified bloom toxin with an irreversible anticholinesterase anatoxin-a(s), produced by A flos-aquae strain NRC-525-17, revealed the bloom toxin and anatoxin-a(s) had similar properties on high-performance liquid chromatography and on the inhibition of electric eel acetylcholinesterase (EC 3.1.1.7).
对与1985年8月26日南达科他州里士满湖9只狗死亡事件有关的蓝藻细菌（蓝绿藻）进行了分析。水样中占主导地位的蓝藻细菌物种是水华鱼腥藻。冻干的水华物质或高效液相色谱法纯化的毒素峰，经腹腔注射给小鼠后，会引发流涎、流泪、尿失禁、排便、抽搐、肌束震颤和呼吸骤停等临床症状。将半纯化的水华毒素与水华鱼腥藻菌株NRC - 525 - 17产生的不可逆抗胆碱酯酶类毒素-a(s) 进一步比较，结果显示，在高效液相色谱分析以及对电鳗乙酰胆碱酯酶（EC 3.1.1.7）的抑制作用方面，水华毒素和类毒素-a(s) 具有相似的特性。
