[医用生物材料上铜绿假单胞菌生物膜的检测]

[Detection of Pseudomonas aeruginosa biofilm on medical biomaterials].

作者信息

Wolska Katarzyna, Jakubczak Antoni

出版信息

Med Dosw Mikrobiol. 2003;55(4):371-8.

Abstract

The adhesion and biofilm formation of Pseudomonas aeruginosa strains on the surface of catheters made of various polymers (PU, SL, PCW) were determined in vitro. It was used the method by Richards et al. with modification of Rózalska et al. (1998), in which soluble colourless TTC is reduced to insoluble red formazan. The results of this study indicate that 80.3% of this strains adhered and 94.6% formed biofilm on the Nelaton catheter, 86% strains adhered and 76.1% formed biofilm on the polyurethane catheter, and 73.2% strains adhered, and 78.9% formed biofilm on the Foley catheter.

摘要

在体外测定了铜绿假单胞菌菌株在由各种聚合物（聚氨酯、硅橡胶、聚碳酸酯）制成的导管表面的黏附及生物膜形成情况。采用了Richards等人的方法，并对Rózalska等人（1998年）的方法进行了改进，其中可溶性无色的氯化三苯基四氮唑被还原为不溶性的红色甲臜。本研究结果表明，80.3%的该菌株在Nelaton导管上黏附，94.6%形成生物膜；86%的菌株在聚氨酯导管上黏附，76.1%形成生物膜；73.2%的菌株在Foley导管上黏附，78.9%形成生物膜。