Kwiecinska-Piróg Joanna, Bogiel Tomasz, Skowron Krzysztof, Wieckowska Ewa, Gospodarek Eugenia
Department of Microbiology Faculty of Pharmacy Ludwik Rydygier Collegium Medicum Nicolaus Copernicus University in Torun Bydgoszcz Poland Department of Microbiology, Faculty of Pharmacy, Ludwik Rydygier Collegium Medicum, Nicolaus Copernicus University in Torun, Bydgoszcz, Poland.
Braz J Microbiol. 2015 Mar 4;45(4):1423-31. doi: 10.1590/s1517-83822014000400037. eCollection 2014.
Proteus mirabilis strains ability to form biofilm is a current topic of a number of research worldwide. In this study the biofilm formation of P. mirabilis strains derived from urine of the catheterized and non-catheterized patients has been investigated. A total number of 39 P. mirabilis strains isolated from the urine samples of the patients of dr Antoni Jurasz University Hospital No. 1 in Bydgoszcz clinics between 2011 and 2012 was used. Biofilm formation was evaluated using two independent quantitative and qualitative methods with TTC (2,3,5-triphenyl-tetrazolium chloride) and CV (crystal violet) application. The obtained results confirmed biofilm formation by all the examined strains, except quantitative method with TTC, in which 7.7% of the strains did not have this ability. It was shown that P. mirabilis rods have the ability to form biofilm on the surfaces of both biomaterials applied, polystyrene and polyvinyl chloride (Nelaton catheters). The differences in ability to form biofilm observed between P. mirabilis strains derived from the urine of the catheterized and non-catheterized patients were not statistically significant.
奇异变形杆菌形成生物膜的能力是当前全球众多研究的主题。在本研究中，对来自留置导管患者和未留置导管患者尿液中的奇异变形杆菌菌株的生物膜形成情况进行了调查。使用了2011年至2012年间从比得哥什第一安托尼·尤拉斯医院临床患者尿液样本中分离出的总共39株奇异变形杆菌菌株。采用两种独立的定量和定性方法，即应用TTC（2,3,5-三苯基氯化四氮唑）和CV（结晶紫）来评估生物膜的形成。所得结果证实，除了使用TTC的定量方法中有7.7%的菌株没有这种能力外，所有检测的菌株都能形成生物膜。结果表明，奇异变形杆菌杆菌能够在应用的两种生物材料表面，即聚苯乙烯和聚氯乙烯（内拉通导管）上形成生物膜。在来自留置导管患者和未留置导管患者尿液的奇异变形杆菌菌株之间观察到的生物膜形成能力差异无统计学意义。
